Talk:20.109(S13):Characterize protein expression (Day7)

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[[Image:S13_M2D7_TR_Orange.JPG | left |thumb|250px | T/R Gel, Orange group]]
[[Image:S13_M2D7_TR_Orange.JPG | left |thumb|250px | T/R Gel, Orange group]]
[[Image:S13_M2D7_TR_Yellow.JPG | center |thumb|250px | T/R Gel, Yellow group -- really Orange]]
[[Image:S13_M2D7_TR_Yellow.JPG | center |thumb|250px | T/R Gel, Yellow group -- really Orange]]
-
[[Image:S13_M2D7_TR_Green.JPG | right |thumb|250px | T/R Gel, Green group]]
+
[[Image:S13_M2D7_TR_Green.JPG | right |thumb|250px | T/R Gel, Green group -- really Yellow]]
[[Image:S13_M2D7_TR_Blue.JPG | left |thumb|250px | T/R Gel, Blue group (broke a little, sorry)]]
[[Image:S13_M2D7_TR_Blue.JPG | left |thumb|250px | T/R Gel, Blue group (broke a little, sorry)]]
[[Image:S13_M2D7_TR_Purple.JPG | center |thumb|250px | T/R Gel, Purple group]]
[[Image:S13_M2D7_TR_Purple.JPG | center |thumb|250px | T/R Gel, Purple group]]

Revision as of 16:08, 18 April 2013

Contents

SDS-PAGE analysis tips

You can look up the expected molecular weight of IPC using the sequence file (from Day 1 or Day 5) and this link or a similar website. Be sure to add ~ 3 KDa for the size of the N-terminus of pRSET (His tag, etc)!

If you see two strong bands on your gel, what do you think the second one is? As a starting hint, look back at the Day 6 protocol and carefully consider what is present in the unpurified protein tubes.

What other assays might you consider in relation to SDS-PAGE in order to take a holistic view of your data?

SDS-PAGE images

T/R

T/R Gel, Red group
T/R Gel, Red group
T/R Gel, Orange group
T/R Gel, Orange group
T/R Gel, Yellow group -- really Orange
T/R Gel, Yellow group -- really Orange
T/R Gel, Green group -- really Yellow
T/R Gel, Green group -- really Yellow
T/R Gel, Blue group (broke a little, sorry)
T/R Gel, Blue group (broke a little, sorry)
T/R Gel, Purple group
T/R Gel, Purple group
T/R Gel, Platinum group
T/R Gel, Platinum group


W/F

W/F Gel, Red group
W/F Gel, Red group
W/F Gel, Orange group
W/F Gel, Orange group
W/F Gel, Yellow group
W/F Gel, Yellow group
W/F Gel, Green group
W/F Gel, Green group
W/F Gel, Blue group
W/F Gel, Blue group
W/F Gel, Pink group
W/F Gel, Pink group
W/F Gel, Purple group
W/F Gel, Purple group
W/F Gel, Platinum group
W/F Gel, Platinum group


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