Talk:20.109(S13):Characterize protein expression (Day7)

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(T/R)
Current revision (16:32, 18 April 2013) (view source)
(W/F)
 
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===T/R===
===T/R===
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[[Image:S13_M2D7_TR_Red.JPG | right |thumb|250px | T/R Gel, Red group]]
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[[Image:S13_M2D7_TR_Red.JPG | right |thumb|250px | T/R Gel, Red group -- really W/F Platinum!]]
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[[Image:S13_M2D7_TR_Orange.JPG | left |thumb|250px | T/R Gel, Orange group]]
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[[Image:S13_M2D7_TR_Orange.JPG | left |thumb|250px | T/R Gel, Orange group -- really Red]]
[[Image:S13_M2D7_TR_Yellow.JPG | center |thumb|250px | T/R Gel, Yellow group -- really Orange]]
[[Image:S13_M2D7_TR_Yellow.JPG | center |thumb|250px | T/R Gel, Yellow group -- really Orange]]
[[Image:S13_M2D7_TR_Green.JPG | right |thumb|250px | T/R Gel, Green group -- really Yellow]]
[[Image:S13_M2D7_TR_Green.JPG | right |thumb|250px | T/R Gel, Green group -- really Yellow]]
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[[Image:S13_M2D7_TR_Blue.JPG | left |thumb|250px | T/R Gel, Blue group (broke a little, sorry)]]
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[[Image:S13_M2D7_TR_Blue.JPG | left |thumb|250px | T/R Gel, Blue group -- really Green]]
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[[Image:S13_M2D7_TR_Purple.JPG | center |thumb|250px | T/R Gel, Purple group]]
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[[Image:S13_M2D7_TR_Purple.JPG | center |thumb|250px | T/R Gel, Purple group -- OK]]
[[Image:S13_M2D7_TR_Platinum.JPG | left |thumb|250px | T/R Gel, Platinum group -- OK]]
[[Image:S13_M2D7_TR_Platinum.JPG | left |thumb|250px | T/R Gel, Platinum group -- OK]]
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<br style="clear:both;"/>
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===W/F===
===W/F===
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[[Image:S13_M2D7_WF_Red.JPG | right |thumb|250px | W/F Gel, Red group]]
+
[[Image:S13_M2D7_WF_Red.JPG | right |thumb|250px | W/F Gel, Red group -- really Orange]]
-
[[Image:S13_M2D7_WF_Orange.JPG | left |thumb|250px | W/F Gel, Orange group]]
+
[[Image:S13_M2D7_WF_Orange.JPG | left |thumb|250px | W/F Gel, Orange group -- really Red]]
-
[[Image:S13_M2D7_WF_Yellow.JPG | center |thumb|250px | W/F Gel, Yellow group]]
+
[[Image:S13_M2D7_WF_Yellow.JPG | center |thumb|250px | W/F Gel, Yellow group -- OK]]
-
[[Image:S13_M2D7_WF_Green.JPG | right |thumb|250px | W/F Gel, Green group]]
+
[[Image:S13_M2D7_WF_Green.JPG | right |thumb|250px | W/F Gel, Green group -- OK]]
-
[[Image:S13_M2D7_WF_Blue.JPG | left |thumb|250px | W/F Gel, Blue group]]
+
[[Image:S13_M2D7_WF_Blue.JPG | left |thumb|250px | W/F Gel, Blue group -- OK]]
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[[Image:S13_M2D7_WF_Pink.JPG | center |thumb|250px | W/F Gel, Pink group]]
+
[[Image:S13_M2D7_WF_Pink.JPG | center |thumb|250px | W/F Gel, Pink group -- really T/R Blue!]]
-
[[Image:S13_M2D7_WF_Purple.JPG | left |thumb|250px | W/F Gel, Purple group]]
+
[[Image:S13_M2D7_WF_Purple.JPG | left |thumb|250px | W/F Gel, Purple group -- really Pink]]
-
[[Image:S13_M2D7_WF_Platinum.JPG | center |thumb|250px | W/F Gel, Platinum group]]
+
[[Image:S13_M2D7_WF_Platinum.JPG | center |thumb|250px | W/F Gel, Platinum group -- really Purple]]
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<br style="clear:both;"/>

Current revision

Contents

SDS-PAGE analysis tips

You can look up the expected molecular weight of IPC using the sequence file (from Day 1 or Day 5) and this link or a similar website. Be sure to add ~ 3 KDa for the size of the N-terminus of pRSET (His tag, etc)!

If you see two strong bands on your gel, what do you think the second one is? As a starting hint, look back at the Day 6 protocol and carefully consider what is present in the unpurified protein tubes.

What other assays might you consider in relation to SDS-PAGE in order to take a holistic view of your data?

SDS-PAGE images

T/R

T/R Gel, Red group -- really W/F Platinum!
T/R Gel, Red group -- really W/F Platinum!
T/R Gel, Orange group -- really Red
T/R Gel, Orange group -- really Red
T/R Gel, Yellow group -- really Orange
T/R Gel, Yellow group -- really Orange
T/R Gel, Green group -- really Yellow
T/R Gel, Green group -- really Yellow
T/R Gel, Blue group -- really Green
T/R Gel, Blue group -- really Green
T/R Gel, Purple group -- OK
T/R Gel, Purple group -- OK
T/R Gel, Platinum group -- OK
T/R Gel, Platinum group -- OK


W/F

W/F Gel, Red group -- really Orange
W/F Gel, Red group -- really Orange
W/F Gel, Orange group -- really Red
W/F Gel, Orange group -- really Red
W/F Gel, Yellow group -- OK
W/F Gel, Yellow group -- OK
W/F Gel, Green group -- OK
W/F Gel, Green group -- OK
W/F Gel, Blue group -- OK
W/F Gel, Blue group -- OK
W/F Gel, Pink group -- really T/R Blue!
W/F Gel, Pink group -- really T/R Blue!
W/F Gel, Purple group -- really Pink
W/F Gel, Purple group -- really Pink
W/F Gel, Platinum group -- really Purple
W/F Gel, Platinum group -- really Purple


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