Talk:20.109(S13):Characterize protein expression (Day7): Difference between revisions
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You can look up the expected molecular weight of IPC using the sequence file (from Day 1 or Day 5) and [http://www.bioinformatics.org/sms/prot_mw.html this link] or a similar website. Be sure to add ~ 3 KDa for the size of the N-terminus of pRSET (His tag, etc)! | You can look up the expected molecular weight of IPC using the sequence file (from Day 1 or Day 5) and [http://www.bioinformatics.org/sms/prot_mw.html this link] or a similar website. Be sure to add ~ 3 KDa for the size of the N-terminus of pRSET (His tag, etc)! | ||
If you see two strong bands on your gel, what do you think the second one is? As a starting hint, look back at the Day 6 protocol and carefully consider what is present in the unpurified protein tubes. | If you see two strong bands on your gel, what do you think the second one is? As a starting hint, look back at the Day 6 protocol and carefully consider what is present in the unpurified protein tubes. | ||
What other assays might you consider in relation to SDS-PAGE in order to take a holistic view of your data? | |||
==SDS-PAGE images== | ==SDS-PAGE images== | ||
Revision as of 12:46, 10 April 2013
SDS-PAGE analysis tips
You can look up the expected molecular weight of IPC using the sequence file (from Day 1 or Day 5) and this link or a similar website. Be sure to add ~ 3 KDa for the size of the N-terminus of pRSET (His tag, etc)!
If you see two strong bands on your gel, what do you think the second one is? As a starting hint, look back at the Day 6 protocol and carefully consider what is present in the unpurified protein tubes.
What other assays might you consider in relation to SDS-PAGE in order to take a holistic view of your data?
SDS-PAGE images
T/R
