Talk:20.109(S13):Assess protein function (Day8)

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(Analysis, W/F)
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|Kd = 0.0916, n = 1.2896
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|Kd = 0.7357, n = 4.3991
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|Kd = 0.6766, n = 7.2874
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|Decreased affinity, increased cooperativity
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Revision as of 20:48, 12 April 2013

Contents

Raw Data

Raw calcium titration data is posted below.


Day 7 data file, T/R Day 7 data file, W/F
Media:S13-M2D7-TR-Red.txt Media:S13-M2D7-WF-Red.txt
Media:S13-M2D7-TR-Org.txt Media:S13-M2D7-WF-Org.txt
Media:S13-M2D7-TR-Ylw.txt Media:S13-M2D7-WF-Ylw.txt
Media:S13-M2D7-TR-Grn.txt Media:S13-M2D7-WF-Grn.txt
Media:S13-M2D7-TR-Blu.txt Media:S13-M2D7-WF-Blu.txt
N/A Media:S13-M2D7-WF-Pnk.txt
Media:S13-M2D7-TR-Prp.txt Media:S13-M2D7-WF-Prp.txt
Media:S13-M2D7-TR-Plt.txt Media:S13-M2D7-WF-Plt.txt


Analysis, T/R

Please be mindful of significant figures when you fill in this table. Do not state far more precise numbers than you can believe.

Group Colour D24H, E67K, T79P, or M124S? X#Z Mutant Most believable KD; n for WT Most believable KD; n for reference Most believable KD; n for X#Z mutant Comments (e.g., very low fluorescence)
Plt M124S D80R WT: 0.0976; 1.4751 M124S: 0.4115; 3.9341 D80R: 1.2991; 2.8465 small difference between 0% and 100% for our mutant, affinity decrease and cooperativity increase
Orange D24H G25Q 0.1462, n = 0.8310 0.6759, n = 2.5734 0.7118; n = -0.2595 Very low fluorescence; oscillating trend...
Purple M124S M144R KD = 0.0672, n = 1.1286 KD = 0.2682, n= 3.3655 KD = 0.3583, n= 4.1618 Higher cooperativity, lower affinity in target mutant
Blue M124S M71R KD = 0.1175, n = 1.4880 KD = 0.3377, n = 3.3863 KD = 0.2246, n = 0.6741 Negative cooperativity, lower affinity in target mutant

Analysis, W/F

Analysis, W/F

Please be mindful of significant figures when you fill in this table. Do not state far more precise numbers than you can believe.

Group Colour D24H, E67K, T79P, or M124S? X#Z Mutant Most believable KD; n for WT Most believable KD; n for reference Most believable KD; n for X#Z mutant Comments (e.g., very low fluorescence)
Blue D24H D22E Kd= 0.0781, n= 1.2420 Kd= 0.5804, n= 2.3194 Kd= 0.6807, n= 7.0509 Affinity decreased while the hill coefficient increased significantly. No extreme outliers.
Platinum E67K R126P Kd = 0.0970 n =1.0639 Kd = 0.3729 n = 0.3830 Kd = 0.3488 n= 0.9085

Low fluorescent values

Pink D24H G25Q Kd = 0.1232, n = 1.3583 Kd = 0.7541, n = 3.9963 Kd = 0.0415, n = -0.4091 Low fluorescence relative to wt, Affinity increased significantly or IPC was unable to refold properly.
Purple M12S M51T Kd=0.1276, n=1.4726 Kd=0.3430, n=3.009 Kd=0.1191, n=1.2544
Yellow M124S M51T Kd = 0.0739; n = 1.2038 Kd = 0.3859; n = 3.0814 Kd = 0.1403; n = 1.6126 Higher fluorescence compared to WT. Similar affinity as WT, higher affinity than reference.
Red D24H D22E Kd = 0.0919, n = 1.858 Kd = 0.7099, n = 6.5600 Kd = 0.6134, n = 2.3619
Orange D24H D22E Kd = 0.0916, n = 1.2896 Kd = 0.7357, n = 4.3991 Kd = 0.6766, n = 7.2874 Decreased affinity, increased cooperativity
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