Talk:20.109(S13):Assess protein function (Day8)

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Analysis, T/R)
Line 68: Line 68:
|Very low fluorescence; oscillating trend...
|Very low fluorescence; oscillating trend...
|-
|-
-
|
+
|Purple
-
|
+
|M124S
-
|
+
|M144R
-
|
+
|KD = 0.0672, n = 1.1286
-
|
+
|KD = 0.2682, n= 3.3655
-
|
+
|KD = 0.3583, n= 4.1618
-
|
+
|Higher cooperativity, lower affinity in target mutant
|-
|-
|
|

Revision as of 15:06, 11 April 2013

Contents

Raw Data

Raw calcium titration data is posted below.


Day 7 data file, T/R Day 7 data file, W/F
Media:S13-M2D7-TR-Red.txt Media:S13-M2D7-WF-Red.txt
Media:S13-M2D7-TR-Org.txt Media:S13-M2D7-WF-Org.txt
Media:S13-M2D7-TR-Ylw.txt Media:S13-M2D7-WF-Ylw.txt
Media:S13-M2D7-TR-Grn.txt Media:S13-M2D7-WF-Grn.txt
Media:S13-M2D7-TR-Blu.txt Media:S13-M2D7-WF-Blu.txt
N/A Media:S13-M2D7-WF-Pnk.txt
Media:S13-M2D7-TR-Prp.txt Media:S13-M2D7-WF-Prp.txt
Media:S13-M2D7-TR-Plt.txt Media:S13-M2D7-WF-Plt.txt


Analysis, T/R

Please be mindful of significant figures when you fill in this table. Do not state far more precise numbers than you can believe.

Group Colour D24H, E67K, T79P, or M124S? X#Z Mutant Most believable KD; n for WT Most believable KD; n for reference Most believable KD; n for X#Z mutant Comments (e.g., very low fluorescence)
Plt M124S D80R WT: 0.0976; 1.4751 M124S: 0.4115; 3.9341 D80R: 1.2991; 2.8465 small difference between 0% and 100% for our mutant, affinity decrease and cooperativity increase
Orange D24H G25Q 0.1462, n = 0.8310 0.6759, n = 2.5734 0.7118; n = -0.2595 Very low fluorescence; oscillating trend...
Purple M124S M144R KD = 0.0672, n = 1.1286 KD = 0.2682, n= 3.3655 KD = 0.3583, n= 4.1618 Higher cooperativity, lower affinity in target mutant

Analysis, W/F

Analysis, T/R

Please be mindful of significant figures when you fill in this table. Do not state far more precise numbers than you can believe.

Group Colour D24H, E67K, T79P, or M124S? X#Z Mutant Most believable KD; n for WT Most believable KD; n for reference Most believable KD; n for X#Z mutant Comments (e.g., very low fluorescence)
Personal tools