Talk:20.109(S13):Assess protein function (Day8)

Raw Data

Raw calcium titration data is posted below.

Day 7 data file, T/R	Day 7 data file, W/F
Media:S13-M2D7-TR-Red.txt	Media:S13-M2D7-WF-Red.txt
Media:S13-M2D7-TR-Org.txt	Media:S13-M2D7-WF-Org.txt
Media:S13-M2D7-TR-Ylw.txt	Media:S13-M2D7-WF-Ylw.txt
Media:S13-M2D7-TR-Grn.txt	Media:S13-M2D7-WF-Grn.txt
Media:S13-M2D7-TR-Blu.txt	Media:S13-M2D7-WF-Blu.txt
N/A	Media:S13-M2D7-WF-Pnk.txt
Media:S13-M2D7-TR-Prp.txt	Media:S13-M2D7-WF-Prp.txt
Media:S13-M2D7-TR-Plt.txt	Media:S13-M2D7-WF-Plt.txt

Analysis, T/R

Please be mindful of significant figures when you fill in this table. Do not state far more precise numbers than you can believe.

Group Colour	D24H, E67K, T79P, or M124S?	X#Z Mutant	Most believable KD; n for WT	Most believable KD; n for reference	Most believable KD; n for X#Z mutant	Comments (e.g., very low fluorescence)
Plt	M124S	D80R	WT: 0.0976; 1.4751	M124S: 0.4115; 3.9341	D80R: 1.2991; 2.8465	small difference between 0% and 100% for our mutant, affinity decrease and cooperativity increase
Orange	D24H	G25Q	0.1462, n = 0.8310	0.6759, n = 2.5734	0.7118; n = -0.2595	Very low fluorescence; oscillating trend...
Purple	M124S	M144R	KD = 0.0672, n = 1.1286	KD = 0.2682, n= 3.3655	KD = 0.3583, n= 4.1618	Higher cooperativity, lower affinity in target mutant
Blue	M124S	M71R	KD = 0.1175, n = 1.4880	KD = 0.3377, n = 3.3863	KD = 0.2246, n = 0.6741	Negative cooperativity, lower affinity in target mutant

Analysis, W/F

Please be mindful of significant figures when you fill in this table. Do not state far more precise numbers than you can believe.

Group Colour

D24H, E67K, T79P, or M124S?

X#Z Mutant

Most believable KD; n for WT

Most believable KD; n for reference

Most believable KD; n for X#Z mutant

Comments (e.g., very low fluorescence)

Blue

D24H

D22E

Kd= 0.0778, n= 1.2080

Kd= 0.6353, n= 3.7325

Kd= 0.6892, n= 7.8230

Affinity decreased while the hill coefficient increased significantly. No extreme outliers.