Talk:20.109(S11):Purify RNA and run affinity column (Day4)

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Revision as of 14:40, 10 February 2011

Sign-up for column protocols

T/R

You and your partner should propose a protocol in the table below.

If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.

Protocol# Group 8-12 % Wash # (partner 1) Wash # (partner 2) Note changes to plan here
0 Sample 5 5 15 Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12.
1 Red .5 4 24 Starting with a lower 8-12 concentration.
2 Orange
3 Yellow 1 4 24 starting with a lower 8-12 concentration
4 Green 50 4 24 Repeating high-percentage run for statistical reliability.
5 Blue
6 Pink 2 4 24 Repeat from last year's experiment
7 Purple


W/F

You and your partner should propose a protocol in the table below.

If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.

Protocol# Group 8-12 % Wash # (partner 1) Wash # (partner 2) Note changes to plan here
0 Sample 5 5 15 Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12.
1 Red
2 Orange
3 Yellow
4 Green
5 Blue
6 Pink
7 Purple


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