Talk:20.109(S09): Luciferase assays and RNA prep (Day6)

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(W/F Luciferase Reactions)
(W/F Luciferase Reactions)
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Revision as of 20:51, 7 April 2009

T/R Luciferase Reactions

Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (Renilla/firefly).

Note: feel free to put ratios from either background-subtracted or raw values; they are likely to be quite similar. Hoewever, if you see this note, please mark which kind of data your ratios come from.

Team Colour none pLuc only validated scrambled experimental raw or subtracted?
Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio
Pink 4.5 4.0 20.7 19.1 2.73 1.92 18.9 22.9 14.7 17.0
Green .94 .97 36.4 26.3 2.7 2.4 27.6 29.1 2.8 2.6 Raw
Orange .076 22.3 21.2 29.0 2.25 1.74 22.0 22.8 4.8 4.1
Yellow 1.33 .17 20.8 29.4 1.42 2.24 25.74 26.08 1.17 1.53
Grey 1.74 1.10 38.64 34.59 2.55 2.90 37.75 35.83 9.04 8.80 Raw
Red 2.02 3.00 21.36 16.40 1.31 1.51 20.78 23.90 1.53 1.61 Raw
Blue 1.57 3.64 14.78 18.67 1.75 .51 17.40 15.16 8.25 6.87
Purple 1.03 1.03 40.4 0.847* (not reliable) 3.24 3.90 23.9 29.8 7.55 8.33 Raw

W/F Luciferase Reactions

Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (Renilla/firefly).

Note: feel free to put ratios from either background-subtracted or raw values; they are likely to be quite similar. Hoewever, if you see this note, please mark which kind of data your ratios come from.

Team Colour none pLuc only validated scrambled experimental raw or subtracted?
Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio
Pink 1.83 1.39 22.59 20.86 2.87 2.91 21.06 21.61 18.10 18.08 R A W
Orange 1.11 0.89 20.56 11.30 1.70 2.30 21.71 16.40 6.42 5.13
Red 2.133 2.6 5.924 14.4 0.385 1.636 20.96 19.89 1.96 1.72
Blue 1.019 0.761658 16.17009 19.39064 2.758433 8.679711 15.43884 10.86473 14.8144 12.87329 raw!
Purple 1.1016 1.820 32.05 30.98 1.369 2.073 35.384 33.298 1.189 1.751 raw
Green 0.878 1.028 31.548 23.173 1.562 3.331 24.934 18.317 4.434 9.589 Raw
Yellow 1.071 0.942 29.831 29.021 3.040 3.575 30.356 31.690 1.989 2.203 RAW
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