Talk:20.109(S09):Data analysis (Day8)

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(W/F data)
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|E31I killed calcium binding
|E31I killed calcium binding
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|M124S
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|.5080 uM, 14.8384
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|.5066 uM, 11.4047
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|.6292 uM, 2.2983
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|M124S increased binding, increased cooperativity (but our WT Kd is suspiciously high)
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Revision as of 22:27, 8 March 2009

T/R data

Below is your calcium titration data, as well as data from pilot experiments done by the teaching faculty for comparison.

Please fill in the second table by Sunday, if not today, so your classmates can compare their data to yours.


Day 7 data file Data taken after 24 hr settling period
Media:S09_M1D7_TeachingExample.xls Mutations: WT, S101L, Y64D
Media:S09_M1D7_TR_Blue.txt
Media:S09_M1D7_TR_Green.txt Media:S09_M1D7_TR_Green-redo.txt
Media:S09_M1D7_TR_Grey.txt Media:S09_M1D7_TR_Grey-redo.txt
Media:S09_M1D7_TR_Orange.txt
Media:S09_M1D7_TR_Pink.txt Media:S09_M1D7_TR_Pink-redo.txt
Media:S09_M1D7_TR_Purple.txt Media:S09_M1D7_TR_Purple-redo.txt
Media:S09_M1D7_TR_Red.txt
Media:S09_M1D7_TR_Yellow.txt


Group Colour X#Z Mutant Most believable KD; n for X#Z mutant Most believable KD; n for S101L Most believable KD; n for WT Comments
Teaching Faculty Y64D N/A ~ 0.5 μM ; ~ 8 ~ 0.3 μM; ~ 1(.2) Y64D killed calcium response
Yellow Y64D N/A ~0.4 μM; ~7 ~0.5 μM; ~3 No Calcium binding in Y64D
Grey D22W N/A 0.46 μM ; ~ 8 0.3 μM; ~ 2 killed calcium binding
Red D131G ~ 0.6 μM ; ~ 10.31 ~ 0.45 μM ; ~ 32.7 ~ 0.3 μM ; ~ 2 D131G altered cooperativity and binding
Green D133W 0.52 μM ; 0.87 0.51 μM ; 12.6 0.51 μM ; 8.9 D133W altered cooperativity but not binding
Orange E31I N/A 0.49 μM ; ~ 7 0.49 μM; ~ 7.6 E31I killed calcium binding
Pink M124S .5080 uM, 14.8384 .5066 uM, 11.4047 .6292 uM, 2.2983 M124S increased binding, increased cooperativity (but our WT Kd is suspiciously high)

W/F data

Below is your calcium titration data, as well as data from pilot experiments done by the teaching faculty for comparison.

Please fill in the second table by Sunday, if not today, so your classmates can compare their data to yours.


Day 7 data file Data taken after 24 hr settling period
Media:S09_M1D7_TeachingExample.xls Mutations: WT, S101L, Y64D
Media:S09_M1D7_WF_Blue.txt
Media:S09_M1D7_WF_Green.txt
Media:S09_M1D7_WF_Grey.txt
Media:S09_M1D7_WF_Orange-1.txt
Media:S09_M1D7_WF_Orange-2.txt
Media:S09_M1D7_WF_Orange-redo.txt
Media:S09_M1D7_WF_Pink.txt
Media:S09_M1D7_WF_Purple-1.txt
Media:S09_M1D7_WF_Purple-2.txt
Media:S09_M1D7_WF_Red.txt
Media:S09_M1D7_WF_Yellow.txt Media:S09_M1D7_WF_Yellow-redo.txt


Group Colour X#Z Mutant Most believable KD; n for X#Z mutant Most believable KD; n for S101L Most believable KD; n for WT Comments
Teaching Faculty Y64D N/A ~ 0.5 μM ; ~ 8 ~ 0.3 μM ; ~ 1(.2) Y64D killed calcium response
Orange E127K Kd-.4558, n-1.2418 N/A (using teaching data) Kd=.6285, n=.1490 S101L and E127K data was noisy
Green T79P Kd~0.84 μM, n~2.5 Kd~0.52 μM, n~7.5 Kd~0.50 μM, n~7.6 T79P seems to have decreased cooperativity
Blue E31R Kd~0.49 μM, n~0.96 Used Teaching Sample: Kd~0.5 μM, n~1.1 Kd~0.45 μM, n~7.4 E31R decreased calcium binding and cooperativity
Yellow L105S Kd~0.50 μM, n~0.141 Kd~0.39 μM, n~0.331 Kd~0.33 μM, n~0.722 L105S and WT-IPC data was noisy. Appears as through L105S decreased cooperativity
Purple T79P Kd~.5909 μM, n~3.9182 Kd~0.2553 μM, n~1.3804 Kd~.5315 μM, n~5.3068 T79P decreased cooperativity, but increased Ca affinity
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