Talk:20.109(F13): Mod 1 Day 5 Examine candidate clones & tissue culture

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Contents

T/R lab

Digest Results

Note: most of these gels were run using loading dye without RNase (except for team Platinum). In Agi's misguided attempt to keep your methods section simple, she made the call to stick with the NEB loading dye instead of our home-brew loading dye -- sorry, all! Most of the gels seem straightforward to interpret: just ignore the very thick small bands of RNA, and note that your other DNA bands may be shifted down very slightly and are also curved. However, in a couple of cases we may re-run digests/gels, and will keep you posted about it. For the teams with unknown enzymes and/or samples (Green, Blue, and Pink), please modify this page and fill in these details. Feel free to provide feedback about how "interpretable" you think your gel is directly to us.

Red(left to right): Uncut, XhoI, BamHI-HF, X+B; Lanes: 1,2,3,4 – C2; 5,6,8,9 – pCX-NNX; 10,11,12,13 – C3; 7, 14: 1kb marker Orange(left to right): Uncut, XhoI, BamHI-HF, X+B; Lanes: 1,2,3,4 – pCX-NNX; 5,6,8,9 – C1; 10,11,12,13 – C2; 7, 14: 1kb marker Yellow(left to right): Uncut, XhoI, BamHI-HF, X+B; Lanes: 1,2,3,4 – pCX-NNX; 5,6,8,9 – C1; 10,11,12,13 – C2; 7, 14: 1kb marker Green(left to right): Uncut, E1, E2, E1+E2; Lanes: 1,2,3,4 – sample 1; 5,6,8,9 – sample 2; 10,11,12,13 – sample 3; 7, 14: 1kb marker Blue(left to right): Uncut, XhoI, BamHI-HF, X+B; Lanes: 1,2,3,4 – sample 2; 5,6,8,9 – sample 3; 10,11,12,13 – pCX-NNX; 7, 14: 1kb marker
Pink(left to right): Uncut, E1, E2, E1+E2; Lanes: 1,2,3,4 – pCX-NNX; 5,6,8,9 – C1; 10,11,12,13 – C2; 7, 14: 1kb marker Purple(left to right): Uncut, XhoI, BamHI-HF, X+B; Lanes: 1,2,3,4 – pCX-NNX; 5,6,8,9 – C1; 10,11,12,13 – C2; 7, 14: 1kb marker Platinum(left to right): Uncut, PvuI, EcoRV, P+E; Lanes: 1,2,3,4 – pCX-NNX; 5,6,8,9 – C1; 10,11,12,13 – C3; 7, 14: 1kb marker White(left to right): Uncut, XhoI, BamHI-HF, X+B; Lanes: 1,2,3,4 – pCX-NNX; 5,6,8,9 – C2; 10,11,12,13 – C3; 7, 14: 1kb marker

W/F lab

Digest Results

Note: Blue and White teams please edit the details for your enzymes :)

Red(left to right): Uncut, EcoRI, NotI, E+N; Lanes: 1,2,3,4 – pCX-NNX; 5,6,8,9 – C1; 10,11,12,13 – C2; 7, 14: 1kb marker Orange(left to right): Uncut, XbaI, XhoI, X+X; Lanes: 1,2,3,4 – pCX-NNX; 5,6,8,9 – C1; 10,11,12,13 – C3; 7, 14: 1kb marker Yellow(left to right): Uncut, SalI, EcoRV, S+E; Lanes: 1,2,3,4 – pCX-NNX; 5,6,8,9 – C1; 10,11,12,13 – C2; 7, 14: 1kb marker Green(left to right): Uncut, SalI, EcoRV, S+E; Lanes: 1,2,3,4 – pCX-NNX; 5,6,8,9 – C1; 10,11,12,13 – C2; 7, 14: 1kb marker
Blue(left to right): Uncut, E1, E2, E1+E2; Lanes: 1,2,3,4 – pCX-NNX; 5,6,8,9 – C2; 10,11,12,13 – C3; 7, 14: 1kb marker Pink(left to right): Uncut, PvuI, BamHI-HF, P+B; Lanes: 1,2,3,4 – pCX-NNX; 5,6,8,9 – C1; 10,11,12,13 – C2; 7, 14: 1kb marker Purple(left to right): Uncut, BamHI-HF, XhoI, B+X; Lanes: 1,2,3,4 – pCX-NNX; 5,6,8,9 – C1; 10,11,12,13 – C3; 7, 14: 1kb marker White(left to right): Uncut, S, E, S+E; Lanes: 1,2,3,4 – pCX-NNX; 5,6,8,9 – C1; 10,11,12,13 – C3; 7, 14: 1kb marker

Ligation Results

Please put your colony count data in the correct row.

Note: "hypothetical data" just shows made-up numbers similar to what you might expect.

Group Colour pCX-EGFP (#) bkb + ins, no lig (#) bkb + lig, no ins (#) bkb + ins, lig 1 (#) bkb + ins, lig 2 (#)
Hypothetical Data 1000 2 20 100 100
Pink 896 3 1 337
Red 1096 0 6 67 100
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