Talk:20.109(F13): Mod 1 Day 5 Examine candidate clones & tissue culture
|Line 83:||Line 83:|
Revision as of 13:22, 30 September 2013
Note: most of these gels were run using loading dye without RNase (except for team Platinum). In Agi's misguided attempt to keep your methods section simple, she made the call to stick with the NEB loading dye instead of our home-brew loading dye -- sorry, all! Most of the gels seem straightforward to interpret: just ignore the very thick small bands of RNA, and note that your other DNA bands may be shifted down very slightly and are also curved. However, in a couple of cases we may re-run digests/gels, and will keep you posted about it. For the teams with unknown enzymes and/or samples (Green, Blue, and Pink), please modify this page and fill in these details. Feel free to provide feedback about how "interpretable" you think your gel is directly to us.
Note: Blue and White teams please edit the details for your enzymes :)
Please put your colony count data in the correct row.
Note: "hypothetical data" just shows made-up numbers similar to what you might expect.
|Group Colour||pCX-EGFP (#)||bkb + ins, no lig (#)||bkb + lig, no ins (#)||bkb + ins, lig 1 (#)||bkb + ins, lig 2 (#)|