Talk:20.109(F13): Mod 1 Day 3 Agarose gel electrophoresis

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search

Shannon K. Alford (Talk | contribs)
(New page: === Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised. === left right<br> <br style=...)
Next diff →

Revision as of 22:47, 17 September 2013

Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.



T/R lab

Here is your recovery gel: Image:TR_F1320109_M1D3_recoverygel.BMP

Top Gel (numbers refer to lanes):

  • 1-2 Pink
  • 3-4 Purple
  • 5 MW
  • 6-7 White
  • 8-9 Blue

Bottom Gel (numbers refer to lanes):

  • 1-2 Orange
  • 3-4 Yellow
  • 5 MW
  • 6-7 Green
  • 8-9 Platinum
  • 10 Red

Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg).

Personal tools