Talk:20.109(F13): Mod 1 Day 3 Agarose gel electrophoresis

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(Pre-purification gels (for reference/posterity))
Current revision (10:34, 24 September 2013) (view source)
 
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==T/R lab==
==T/R lab==
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===Recovery gel===
Here is your recovery gel:
Here is your recovery gel:
[[Image:TR_F1320109_M1D3_recoverygel.BMP]]
[[Image:TR_F1320109_M1D3_recoverygel.BMP]]
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Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg).
Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg).
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===Pre-purification gels (for reference/posterity)===
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Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!
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[[Image:F13_M1D3_TR-Red.jpg|250px|thumb|right| Red Group]]
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[[Image:F13_M1D3_TR-Org.jpg|250px|thumb|left| Orange Group]]
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[[Image:F13_M1D3_TR-Ylw.jpg|250px|thumb|center| Yellow Group]]
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[[Image:F13_M1D3_TR-Grn.jpg|250px|thumb|right| Green Group]]
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[[Image:Mod1Day3AgaroseGel1 copy.jpg|250px|thumb|left| Blue Group]]
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[[Image:F13_M1D3_TR-Pnk.jpg|250px|thumb|center| Pink Group]]
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[[Image:F13_M1D3_TR-Prp.jpg|250px|thumb|right| Purple Group]]
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[[Image:F13_M1D3_TR-Sil.jpg|250px|thumb|left| Silver Group]]
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[[Image:F13_M1D3_TR-Whi.jpg|250px|thumb|center| White Group]]
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<br style="clear:both;"/>
==W/F lab==
==W/F lab==
===Recovery gel===
===Recovery gel===
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[[Image:F13_M1D3_WF-recovery.jpg|550px|thumb|center| W/F Recovery Gel]]
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Top Gel (numbers refer to lanes):
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*1-2 Red
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*3-4 Orange
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*5 MW
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*6-7 Yellow
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*8-9 Green
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Bottom Gel (numbers refer to lanes):
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*1-2 Blue
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*3-4 Pink
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*5 MW
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*6-7 Purple
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*8-9 White
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Note: We loaded all of the solution that you left for us, and 20 &mu;L of MW marker was loaded (for a total 1.0 &mu;g).
===Pre-purification gels (for reference/posterity)===
===Pre-purification gels (for reference/posterity)===

Current revision

Contents

Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.



T/R lab

Recovery gel

Here is your recovery gel: Image:TR_F1320109_M1D3_recoverygel.BMP

Top Gel (numbers refer to lanes):

  • 1-2 Pink
  • 3-4 Purple
  • 5 MW
  • 6-7 White
  • 8-9 Blue

Bottom Gel (numbers refer to lanes):

  • 1-2 Orange
  • 3-4 Yellow
  • 5 MW
  • 6-7 Green
  • 8-9 Platinum
  • 10 Red

Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg).

Pre-purification gels (for reference/posterity)

Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!

Red Group
Red Group
Orange Group
Orange Group
Yellow Group
Yellow Group
Green Group
Green Group
Blue Group
Blue Group
Pink Group
Pink Group
Purple Group
Purple Group
Silver Group
Silver Group
White Group
White Group


W/F lab

Recovery gel

W/F Recovery Gel
W/F Recovery Gel

Top Gel (numbers refer to lanes):

  • 1-2 Red
  • 3-4 Orange
  • 5 MW
  • 6-7 Yellow
  • 8-9 Green

Bottom Gel (numbers refer to lanes):

  • 1-2 Blue
  • 3-4 Pink
  • 5 MW
  • 6-7 Purple
  • 8-9 White


Note: We loaded all of the solution that you left for us, and 20 μL of MW marker was loaded (for a total 1.0 μg).

Pre-purification gels (for reference/posterity)

Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!

Red Group
Red Group
Orange Group
Orange Group
Yellow Group
Yellow Group
Green Group
Green Group
Blue Group
Blue Group
Pink Group
Pink Group
Purple Group
Purple Group
White Group
White Group
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