Talk:20.109(F13): Mod 1 Day 3 Agarose gel electrophoresis

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(New page: === Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised. === left right<br> <br style=...)
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=== Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised. ===
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== Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised. ==
[[Image:FragsMod1.jpg|left]]
[[Image:FragsMod1.jpg|left]]
[[Image:Excisedfrag.jpg|right]]<br>
[[Image:Excisedfrag.jpg|right]]<br>
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<br style="clear:both" />
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=T/R lab=
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==T/R lab==
Here is your recovery gel:
Here is your recovery gel:
[[Image:TR_F1320109_M1D3_recoverygel.BMP]]
[[Image:TR_F1320109_M1D3_recoverygel.BMP]]
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Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 &mu;L of the solution that you left for us and 20 &mu;L of MW marker was loaded (for a total 1.0 &mu;g).
Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 &mu;L of the solution that you left for us and 20 &mu;L of MW marker was loaded (for a total 1.0 &mu;g).
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==W/F lab==
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===Recovery gel===
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===Pre-purification gels (for reference/posterity)===

Revision as of 15:04, 18 September 2013

Contents

Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.



T/R lab

Here is your recovery gel: Image:TR_F1320109_M1D3_recoverygel.BMP

Top Gel (numbers refer to lanes):

  • 1-2 Pink
  • 3-4 Purple
  • 5 MW
  • 6-7 White
  • 8-9 Blue

Bottom Gel (numbers refer to lanes):

  • 1-2 Orange
  • 3-4 Yellow
  • 5 MW
  • 6-7 Green
  • 8-9 Platinum
  • 10 Red

Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg).

W/F lab

Recovery gel

Pre-purification gels (for reference/posterity)

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