Talk:20.109(F12): Mod 1 Day 7 FACS analysis: Difference between revisions
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| Line 28: | Line 28: | ||
|Orange | |Orange | ||
|block II = 1:30-3PM | |block II = 1:30-3PM | ||
| | |0.00 | ||
| | |58.46 | ||
| | |0.00 | ||
| | |0.01 | ||
| | |2.05 | ||
| | |2.11 | ||
| | |1.96 | ||
| | |1.42 (2:1 ratio) | ||
|- | |- | ||
|Yellow | |Yellow | ||
|block I = 12:30-2PM | |block I = 12:30-2PM | ||
| | |0.06 | ||
| | |66.60 | ||
| | |0.00 | ||
| | |0.01 | ||
| | |1.40 | ||
| | |2.11 | ||
| | |1.20 | ||
| | |1.75 (2 delta5 :1 delta3) | ||
|- | |- | ||
|Green | |Green | ||
| Line 61: | Line 61: | ||
|Blue | |Blue | ||
|Block II - 1:30-3pm | |Block II - 1:30-3pm | ||
| | |0.00 | ||
| | |56.19 | ||
| | |0.00 | ||
| | |0.01 | ||
| | |1.14 | ||
| | |1.61 | ||
| | |1.17 | ||
| | |1:20 ratio--0.62 | ||
|- | |- | ||
|Pink | |Pink | ||
|block I = 12:30-2PM | |block I = 12:30-2PM | ||
| | |0.00 | ||
| | |56.14 | ||
| | |0.00 | ||
| | |0.00 | ||
| | |1.91 | ||
| | |2.51 | ||
| | |0.36 | ||
| | |2.07 (10:1 ratio) | ||
|- | |- | ||
|Purple | |Purple | ||
| Line 141: | Line 141: | ||
|- | |- | ||
|Green | |Green | ||
|Block III - 2:30-4pm | |Block III - 2:30-4pm | ||
| | |000 | ||
| | |52.35 | ||
| | |0.00 | ||
| | |0.00 | ||
| | |1.20 | ||
| | |1.66 | ||
| | |0.97 | ||
| | |0.88 "2/3:4/3" (1:2 with the same amount of DNA as 1:1) | ||
|- | |- | ||
|Blue | |Blue | ||
Latest revision as of 06:59, 11 October 2012
Sign up for time on the FACS. Please arrive at the time you choose and then expect to spend 45 minutes in TC and 45 minutes at FACS. TWO or THREE teams should sign up for each time slot.
T/R FACS schedule
| team color | FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM) | mock data | EGFP data | delta 5 data | delta 3 data | delta5:delta3 (1:1) | delta5:delta3(1:2) | delta5:delta3(1:10) | extra experiment (specify below) |
| Red | block I = 12:30-2PM | 0.02 | 55.81 | 0.00 | 0.00 | 1.46 | 2.08 | 1.45 | 1.40 (1:1 ratio with 10' incubation time) |
| Orange | block II = 1:30-3PM | 0.00 | 58.46 | 0.00 | 0.01 | 2.05 | 2.11 | 1.96 | 1.42 (2:1 ratio) |
| Yellow | block I = 12:30-2PM | 0.06 | 66.60 | 0.00 | 0.01 | 1.40 | 2.11 | 1.20 | 1.75 (2 delta5 :1 delta3) |
| Green | Block III - 2:30-4pm | 0.00 | 50.75 | 0.00 | 0.04 | 1.65 | 2.69 | 1.06 | 1:1 ratio with 10min incubation time - 1.23 |
| Blue | Block II - 1:30-3pm | 0.00 | 56.19 | 0.00 | 0.01 | 1.14 | 1.61 | 1.17 | 1:20 ratio--0.62 |
| Pink | block I = 12:30-2PM | 0.00 | 56.14 | 0.00 | 0.00 | 1.91 | 2.51 | 0.36 | 2.07 (10:1 ratio) |
| Purple | Block II = 1:30-3PM | 0.00 | 50.36 | 0.00 | 0.04 | 2.01 | 2.65 | 1.27 | 2.91 at 2:1 ratio (5:3) |
W/F FACS schedule
| team color | FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM) | mock data | EGFP data | delta 5 data | delta 3 data | delta5:delta3 (1:1) | delta5:delta3 (1:2) | delta5:delta3 (1:10) | extra experiment (specify below) | |
| Purple | Block I = 12:30-2:00pm | 0 | 64.96 | 0 | 0.01 | 1.58 | 3.46 | 1.78 | 1:5 ratio - 3.21 | |
| Orange | ||||||||||
| Yellow | ||||||||||
| Green | Block III - 2:30-4pm | 000 | 52.35 | 0.00 | 0.00 | 1.20 | 1.66 | 0.97 | 0.88 "2/3:4/3" (1:2 with the same amount of DNA as 1:1) | |
| Blue | Block II= 1:30-3pm | 0 | 24.39 | 0 | 0 | 0.65 | 0.84 | 0.36 (used 1:5 ratio) | 2:1 ratio: 0.19 | |
| Pink | Block II = 1:30-3PM | 0 | 62.84 | 0 | 0.01 | 1.30 | 2.02 | 0.64 | 1:1 ratio with higher concentration (0.15ug each): 1.61% |
