Talk:20.109(F12): Mod 1 Day 7 FACS analysis

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Current revision (08:59, 11 October 2012) (view source)
 
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Line 11: Line 11:
|delta 3 data
|delta 3 data
|delta5:delta3 (1:1)
|delta5:delta3 (1:1)
-
|delta5:delta3(>1:1)
+
|delta5:delta3(1:2)
-
|delta5:delta3(<1:1)
+
|delta5:delta3(1:10)
|extra experiment (specify below)
|extra experiment (specify below)
|-
|-
|Red
|Red
|block I = 12:30-2PM
|block I = 12:30-2PM
-
|
+
|0.02
-
|
+
|55.81
-
|
+
|0.00
-
|
+
|0.00
-
|
+
|1.46
-
|
+
|2.08
-
|
+
|1.45
-
|
+
|1.40 (1:1 ratio with 10' incubation time)
|-
|-
|Orange
|Orange
-
|
+
|block II = 1:30-3PM
-
|
+
|0.00
-
|
+
|58.46
-
|
+
|0.00
-
|
+
|0.01
-
|
+
|2.05
-
|
+
|2.11
-
|
+
|1.96
-
|
+
|1.42 (2:1 ratio)
|-
|-
|Yellow
|Yellow
|block I = 12:30-2PM
|block I = 12:30-2PM
-
|
+
|0.06
-
|
+
|66.60
-
|
+
|0.00
-
|
+
|0.01
-
|
+
|1.40
-
|
+
|2.11
-
|
+
|1.20
-
|
+
|1.75 (2 delta5 :1 delta3)
|-
|-
|Green
|Green
-
|
+
|Block III - 2:30-4pm
-
|
+
|0.00
-
|
+
|50.75
-
|
+
|0.00
-
|
+
|0.04
-
|
+
|1.65
-
|
+
|2.69
-
|
+
|1.06
-
|
+
|1:1 ratio with 10min incubation time - 1.23
|-
|-
|Blue
|Blue
|Block II - 1:30-3pm
|Block II - 1:30-3pm
-
|
+
|0.00
-
|
+
|56.19
-
|
+
|0.00
-
|
+
|0.01
-
|
+
|1.14
-
|
+
|1.61
-
|
+
|1.17
-
|
+
|1:20 ratio--0.62
|-
|-
|Pink
|Pink
|block I = 12:30-2PM
|block I = 12:30-2PM
-
|
+
|0.00
-
|
+
|56.14
-
|
+
|0.00
-
|
+
|0.00
-
|
+
|1.91
-
|
+
|2.51
-
|
+
|0.36
-
|
+
|2.07 (10:1 ratio)
|-
|-
|Purple
|Purple
-
|
+
|Block II = 1:30-3PM
-
|
+
|0.00
-
|
+
|50.36
-
|
+
|0.00
-
|
+
|0.04
-
|
+
|2.01
-
|
+
|2.65
-
|
+
|1.27
-
|
+
|2.91 at 2:1 ratio (5:3)
|}
|}
Line 103: Line 103:
|delta 3 data
|delta 3 data
|delta5:delta3 (1:1)
|delta5:delta3 (1:1)
-
|delta5:delta3(>1:1)
+
|delta5:delta3 (1:2)
-
|delta5:delta3(<1:1)
+
|delta5:delta3 (1:10)
|extra experiment (specify below)
|extra experiment (specify below)
|-
|-
|Purple
|Purple
-
|Block I = 12:30-2:30pm
+
|Block I = 12:30-2:00pm
-
|
+
|0
-
|
+
|64.96
-
|
+
|0
-
|
+
|0.01
-
|
+
|1.58
-
|
+
|3.46
-
|
+
|1.78
-
|
+
|1:5 ratio - 3.21
|-
|-
|Orange
|Orange
Line 141: Line 141:
|-
|-
|Green
|Green
-
||
+
|Block III - 2:30-4pm
-
|
+
|000
-
|
+
|52.35
-
|
+
|0.00
-
|
+
|0.00
-
|
+
|1.20
-
|
+
|1.66
-
|
+
|0.97
-
|
+
|0.88 "2/3:4/3" (1:2 with the same amount of DNA as 1:1)
|-
|-
|Blue
|Blue
-
|
+
|Block II= 1:30-3pm
-
|
+
|0
-
|
+
|24.39
-
|
+
|0
-
|
+
|0
-
|
+
|0.65
-
|
+
|0.84
-
|
+
|0.36 (used 1:5 ratio)
-
|
+
|2:1 ratio: 0.19
|-
|-
|Pink
|Pink
-
|
+
|Block II = 1:30-3PM
-
|
+
|0
-
|
+
|62.84
-
|
+
|0
-
|
+
|0.01
-
|
+
|1.30
-
|
+
|2.02
-
|
+
|0.64
 +
|1:1 ratio with higher concentration (0.15ug each): 1.61%
|
|
|}
|}

Current revision

Sign up for time on the FACS. Please arrive at the time you choose and then expect to spend 45 minutes in TC and 45 minutes at FACS. TWO or THREE teams should sign up for each time slot.

T/R FACS schedule

NOTE: data is %gated R2. If you'd like to know #s of cells assayed, please contact group directly.
team color FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM) mock data EGFP data delta 5 data delta 3 data delta5:delta3 (1:1) delta5:delta3(1:2) delta5:delta3(1:10) extra experiment (specify below)
Red block I = 12:30-2PM 0.02 55.81 0.00 0.00 1.46 2.08 1.45 1.40 (1:1 ratio with 10' incubation time)
Orange block II = 1:30-3PM 0.00 58.46 0.00 0.01 2.05 2.11 1.96 1.42 (2:1 ratio)
Yellow block I = 12:30-2PM 0.06 66.60 0.00 0.01 1.40 2.11 1.20 1.75 (2 delta5 :1 delta3)
Green Block III - 2:30-4pm 0.00 50.75 0.00 0.04 1.65 2.69 1.06 1:1 ratio with 10min incubation time - 1.23
Blue Block II - 1:30-3pm 0.00 56.19 0.00 0.01 1.14 1.61 1.17 1:20 ratio--0.62
Pink block I = 12:30-2PM 0.00 56.14 0.00 0.00 1.91 2.51 0.36 2.07 (10:1 ratio)
Purple Block II = 1:30-3PM 0.00 50.36 0.00 0.04 2.01 2.65 1.27 2.91 at 2:1 ratio (5:3)

W/F FACS schedule

NOTE: data is %gated R2. If you'd like to know #s of cells assayed, please contact group directly.
team color FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM) mock data EGFP data delta 5 data delta 3 data delta5:delta3 (1:1) delta5:delta3 (1:2) delta5:delta3 (1:10) extra experiment (specify below)
Purple Block I = 12:30-2:00pm 0 64.96 0 0.01 1.58 3.46 1.78 1:5 ratio - 3.21
Orange
Yellow
Green Block III - 2:30-4pm 000 52.35 0.00 0.00 1.20 1.66 0.97 0.88 "2/3:4/3" (1:2 with the same amount of DNA as 1:1)
Blue Block II= 1:30-3pm 0 24.39 0 0 0.65 0.84 0.36 (used 1:5 ratio) 2:1 ratio: 0.19
Pink Block II = 1:30-3PM 0 62.84 0 0.01 1.30 2.02 0.64 1:1 ratio with higher concentration (0.15ug each): 1.61%
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