Talk:20.109(S15): Complete Western and prepare damaged DNA (Day3): Difference between revisions
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==Repeat DNA damage digest gel== | |||
[[Image:20109(S15) DNA damage digest repeat.png|thumb|left|400px|DNA damage digests repeat]] | |||
Lanes:<br> | |||
1. 1kb ladder<br> | |||
2. PmeI digested pMax-BFP-MCS<br> | |||
3. KpnI digested pMax-BFP-MCS<br> | |||
4. EcoRI/EcoRV digested pMax-BFP-MCS<br> | |||
5. EcoRV/PstI digested pMax-BFP-MCS<br> | |||
6. BglII/EcoRI digested pMax-BFP-MCS<br> | |||
7. BglII/PstI digested pMax-BFP-MCS<br> | |||
8. XbaI digested pMax-BFP-MCS<br> | |||
<br style="clear:both;"/> | |||
==T/R Electrophoresis gels and Western Blots== | ==T/R Electrophoresis gels and Western Blots== | ||
[[Image:TR_M2D3_gel1|thumb|right|200px|Yellow, Green, Blue]] | [[Image:TR_M2D3_gel1|thumb|right|200px|Yellow, Green, Blue]] | ||
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[[Image:purpleTR_reblot.tif|thumb|left|200px|Purple_TR reblot]] | [[Image:purpleTR_reblot.tif|thumb|left|200px|Purple_TR reblot]] | ||
<br style="clear:both;"/> | |||
==W/F Electrophoresis gels and Western Blots== | ==W/F Electrophoresis gels and Western Blots== |
Latest revision as of 11:02, 28 March 2015
Repeat DNA damage digest gel
Lanes:
1. 1kb ladder
2. PmeI digested pMax-BFP-MCS
3. KpnI digested pMax-BFP-MCS
4. EcoRI/EcoRV digested pMax-BFP-MCS
5. EcoRV/PstI digested pMax-BFP-MCS
6. BglII/EcoRI digested pMax-BFP-MCS
7. BglII/PstI digested pMax-BFP-MCS
8. XbaI digested pMax-BFP-MCS
T/R Electrophoresis gels and Western Blots