Talk:20.109(S15): Complete Western and prepare damaged DNA (Day3): Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
(7 intermediate revisions by 2 users not shown) | |||
Line 1: | Line 1: | ||
==Repeat DNA damage digest gel== | |||
[[Image:20109(S15) DNA damage digest repeat.png|thumb|left|400px|DNA damage digests repeat]] | |||
Lanes:<br> | |||
1. 1kb ladder<br> | |||
2. PmeI digested pMax-BFP-MCS<br> | |||
3. KpnI digested pMax-BFP-MCS<br> | |||
4. EcoRI/EcoRV digested pMax-BFP-MCS<br> | |||
5. EcoRV/PstI digested pMax-BFP-MCS<br> | |||
6. BglII/EcoRI digested pMax-BFP-MCS<br> | |||
7. BglII/PstI digested pMax-BFP-MCS<br> | |||
8. XbaI digested pMax-BFP-MCS<br> | |||
<br style="clear:both;"/> | |||
==T/R Electrophoresis gels and Western Blots== | ==T/R Electrophoresis gels and Western Blots== | ||
[[Image:TR_M2D3_gel1|thumb|right|200px|Yellow, Green, Blue]] | [[Image:TR_M2D3_gel1|thumb|right|200px|Yellow, Green, Blue]] | ||
[[Image:TR_M2D3_gel2|thumb|left|200px|Orange, Purple]] | [[Image:TR_M2D3_gel2|thumb|left|200px|Orange, Purple]] | ||
[[Image:T_Th YELLOW.tif|thumb|left| | [[Image:T_Th YELLOW.tif|thumb|left|200px|Yellow_TR]] | ||
[[Image:YellowTR_reblot.tif|thumb|center| | [[Image:YellowTR_reblot.tif|thumb|center|200px|Yellow_TR reblot]] | ||
[[Image:T_Th GREEN.tif|thumb|right| | [[Image:T_Th GREEN.tif|thumb|right|200px|Green_TR]] | ||
[[Image:GreenTR_reblot.tif|thumb|left| | [[Image:GreenTR_reblot.tif|thumb|left|200px|Green_TR reblot]] | ||
[[Image:T_Th BLUE.tif|thumb|center| | [[Image:T_Th BLUE.tif|thumb|center|200px|Blue_TR]] | ||
[[Image:blueTR_reblot.tif|thumb|right| | [[Image:blueTR_reblot.tif|thumb|right|200px|Blue_TR reblot]] | ||
[[Image:T_Th ORANGE.tif|thumb|left| | [[Image:T_Th ORANGE.tif|thumb|left|200px|Orange_TR]] | ||
[[Image:orangeTR_reblot.tif|thumb|center| | [[Image:orangeTR_reblot.tif|thumb|center|200px|Orange_TR reblot]] | ||
[[Image:T_Th PURPLE.tif|thumb|right| | [[Image:T_Th PURPLE.tif|thumb|right|200px|Purple_TR]] | ||
[[Image:purpleTR_reblot.tif|thumb|left| | [[Image:purpleTR_reblot.tif|thumb|left|200px|Purple_TR reblot]] | ||
<br style="clear:both;"/> | |||
==W/F Electrophoresis gels and Western Blots== | ==W/F Electrophoresis gels and Western Blots== | ||
Line 19: | Line 36: | ||
[[Image:KU002109.jpg|thumb|left|200px| yellow, red]] | [[Image:KU002109.jpg|thumb|left|200px| yellow, red]] | ||
[[Image:W_F YELLOW.tif|thumb|left|200px|Yellow_WF]] | [[Image:W_F YELLOW.tif|thumb|left|200px|Yellow_WF]] | ||
[[Image:W_F GREEN.tif|thumb|center|200px|Green_WF]] | [[Image:yellowWF_reblot1.tif|thumb|center|200px|Yellow_WF reblot scan 1]] | ||
[[Image:yellowWF_reblot2.tif|thumb|right|200px|Yellow_WF reblot scan 2]] | |||
[[Image:W_F GREEN.tif|thumb|left|200px|Green_WF]] | |||
[[Image:greenwf_reblot.tif|thumb|center|200px|Green_WF reblot]] | |||
[[Image:W_F BLUE.tif|thumb|right|200px|Blue_WF]] | [[Image:W_F BLUE.tif|thumb|right|200px|Blue_WF]] | ||
[[Image:W_F ORANGE.tif|thumb| | [[Image:blueWF_reblot.tif|thumb|left|200px|Blue_WF reblot]] | ||
[[Image:W_F PURPLE.tif|thumb|center|200px|Purple_WF]] | [[Image:W_F ORANGE.tif|thumb|center|200px|Orange_WF]] | ||
[[Image:orangeWF_reblot.tif|thumb|right|200px|Orange_WF reblot]] | |||
[[Image:W_F PURPLE.tif|thumb|left|200px|Purple_WF]] | |||
[[Image:purpleWF_reblot.tif|thumb|center|200px|Purple_WF reblot]] | |||
[[Image:W_F PINK.tif|thumb|right|200px|Pink_WF]] | [[Image:W_F PINK.tif|thumb|right|200px|Pink_WF]] | ||
[[Image:W_F PLATINUM.tif|thumb| | [[Image:pinkWF_reblot.tif|thumb|left|200px|Pink_WF reblot]] | ||
[[Image:W_F RED.tif|thumb|center|200px|Red_WF]] | [[Image:W_F PLATINUM.tif|thumb|center|200px|Platinum_WF]] | ||
[[Image:platinumWF_reblot.tif|thumb|right|200px|Platinum_WF reblot]] | |||
[[Image:W_F RED.tif|thumb|left|200px|Red_WF]] | |||
[[Image:RedWF_reblot.tif|thumb|center|200px|Red_WF reblot]] |
Latest revision as of 11:02, 28 March 2015
Repeat DNA damage digest gel
Lanes:
1. 1kb ladder
2. PmeI digested pMax-BFP-MCS
3. KpnI digested pMax-BFP-MCS
4. EcoRI/EcoRV digested pMax-BFP-MCS
5. EcoRV/PstI digested pMax-BFP-MCS
6. BglII/EcoRI digested pMax-BFP-MCS
7. BglII/PstI digested pMax-BFP-MCS
8. XbaI digested pMax-BFP-MCS
T/R Electrophoresis gels and Western Blots