Talk:20.109(S15): Complete Western and prepare damaged DNA (Day3): Difference between revisions
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=== | ==Repeat DNA damage digest gel== | ||
[[Image:20109(S15) DNA damage digest repeat.png|thumb|left|400px|DNA damage digests repeat]] | |||
[[Image: | |||
Lanes:<br> | |||
1. 1kb ladder<br> | |||
2. PmeI digested pMax-BFP-MCS<br> | |||
3. KpnI digested pMax-BFP-MCS<br> | |||
4. EcoRI/EcoRV digested pMax-BFP-MCS<br> | |||
5. EcoRV/PstI digested pMax-BFP-MCS<br> | |||
6. BglII/EcoRI digested pMax-BFP-MCS<br> | |||
7. BglII/PstI digested pMax-BFP-MCS<br> | |||
8. XbaI digested pMax-BFP-MCS<br> | |||
<br> | <br style="clear:both;"/> | ||
===W/F Electrophoresis gels and Western Blots | ==T/R Electrophoresis gels and Western Blots== | ||
[[Image:KU002107.jpg|thumb|right| | [[Image:TR_M2D3_gel1|thumb|right|200px|Yellow, Green, Blue]] | ||
[[Image:KU002108.jpg|thumb| | [[Image:TR_M2D3_gel2|thumb|left|200px|Orange, Purple]] | ||
[[Image:KU002109.jpg|thumb| | [[Image:T_Th YELLOW.tif|thumb|left|200px|Yellow_TR]] | ||
[[Image:YellowTR_reblot.tif|thumb|center|200px|Yellow_TR reblot]] | |||
[[Image:T_Th GREEN.tif|thumb|right|200px|Green_TR]] | |||
[[Image:GreenTR_reblot.tif|thumb|left|200px|Green_TR reblot]] | |||
[[Image:T_Th BLUE.tif|thumb|center|200px|Blue_TR]] | |||
[[Image:blueTR_reblot.tif|thumb|right|200px|Blue_TR reblot]] | |||
[[Image:T_Th ORANGE.tif|thumb|left|200px|Orange_TR]] | |||
[[Image:orangeTR_reblot.tif|thumb|center|200px|Orange_TR reblot]] | |||
[[Image:T_Th PURPLE.tif|thumb|right|200px|Purple_TR]] | |||
[[Image:purpleTR_reblot.tif|thumb|left|200px|Purple_TR reblot]] | |||
<br style="clear:both;"/> | |||
==W/F Electrophoresis gels and Western Blots== | |||
[[Image:KU002107.jpg|thumb|right|200px| Platinum, Blue Green]] | |||
[[Image:KU002108.jpg|thumb|center|200px| Pink, Orange,Purple]] | |||
[[Image:KU002109.jpg|thumb|left|200px| yellow, red]] | |||
[[Image:W_F YELLOW.tif|thumb|left|200px|Yellow_WF]] | |||
[[Image:yellowWF_reblot1.tif|thumb|center|200px|Yellow_WF reblot scan 1]] | |||
[[Image:yellowWF_reblot2.tif|thumb|right|200px|Yellow_WF reblot scan 2]] | |||
[[Image:W_F GREEN.tif|thumb|left|200px|Green_WF]] | |||
[[Image:greenwf_reblot.tif|thumb|center|200px|Green_WF reblot]] | |||
[[Image:W_F BLUE.tif|thumb|right|200px|Blue_WF]] | |||
[[Image:blueWF_reblot.tif|thumb|left|200px|Blue_WF reblot]] | |||
[[Image:W_F ORANGE.tif|thumb|center|200px|Orange_WF]] | |||
[[Image:orangeWF_reblot.tif|thumb|right|200px|Orange_WF reblot]] | |||
[[Image:W_F PURPLE.tif|thumb|left|200px|Purple_WF]] | |||
[[Image:purpleWF_reblot.tif|thumb|center|200px|Purple_WF reblot]] | |||
[[Image:W_F PINK.tif|thumb|right|200px|Pink_WF]] | |||
[[Image:pinkWF_reblot.tif|thumb|left|200px|Pink_WF reblot]] | |||
[[Image:W_F PLATINUM.tif|thumb|center|200px|Platinum_WF]] | |||
[[Image:platinumWF_reblot.tif|thumb|right|200px|Platinum_WF reblot]] | |||
[[Image:W_F RED.tif|thumb|left|200px|Red_WF]] | |||
[[Image:RedWF_reblot.tif|thumb|center|200px|Red_WF reblot]] | |||
Latest revision as of 11:02, 28 March 2015
Repeat DNA damage digest gel
_DNA_damage_digest_repeat.png)
Lanes:
1. 1kb ladder
2. PmeI digested pMax-BFP-MCS
3. KpnI digested pMax-BFP-MCS
4. EcoRI/EcoRV digested pMax-BFP-MCS
5. EcoRV/PstI digested pMax-BFP-MCS
6. BglII/EcoRI digested pMax-BFP-MCS
7. BglII/PstI digested pMax-BFP-MCS
8. XbaI digested pMax-BFP-MCS
T/R Electrophoresis gels and Western Blots
W/F Electrophoresis gels and Western Blots
