Talk:20.109(S15):Begin Western protein analysis and choose system conditions (Day2): Difference between revisions
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(New page: Please include both your section and your team color in one slot below. Across the class, be sure that each topology is tested by at least '''two''' groups. {| border="1" ! Cut topology !...) |
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Please include both your section and your team color in one slot below. Across the class, be sure that each topology is tested by at least | Please include both your section and your team color in one slot below. Across the class, be sure that each topology is tested by at least one group. | ||
{| border="1" | {| border="1" | ||
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! Group 1 | ! Group 1 | ||
! Group 2 | ! Group 2 | ||
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| Sticky ends, 5' overhangs | | Sticky ends, 5' overhangs | ||
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|- | |- | ||
| Sticky ends, 3' overhangs | | Sticky ends, 3' overhangs | ||
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|- | |- | ||
| Blunt | | Blunt | ||
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|- | |- | ||
| Hybrid: 5' sticky upstream, blunt downstream | | Hybrid: 5' sticky upstream, blunt downstream | ||
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|- | |- | ||
| Hybrid: 3' sticky upstream, blunt downstream | | Hybrid: 3' sticky upstream, blunt downstream | ||
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|- | |- | ||
| Sticky ends with sequence mismatch | | Sticky ends with sequence mismatch | ||
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|- | |- | ||
| Sticky ends with sequence and topology mismatch | | Sticky ends with sequence and topology mismatch | ||
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|} | |} | ||
Revision as of 19:39, 16 March 2015
Please include both your section and your team color in one slot below. Across the class, be sure that each topology is tested by at least one group.
| Cut topology | Group 1 | Group 2 |
|---|---|---|
| Sticky ends, 5' overhangs | ||
| Sticky ends, 3' overhangs | ||
| Blunt | ||
| Hybrid: 5' sticky upstream, blunt downstream | ||
| Hybrid: 3' sticky upstream, blunt downstream | ||
| Sticky ends with sequence mismatch | ||
| Sticky ends with sequence and topology mismatch |
