Talk:20.109(S14):Transcript-level analysis (Day6): Difference between revisions
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==W/F PG== | |||
[[Media:S14_M3D6-WF-PG-Red-Org.txt]] <br> | |||
[[Media:S14_M3D6-WF-PG-Ylw-Grn.txt]] <br> | |||
==T/R pairings== | ==T/R pairings== | ||
[[Image:S14_research_disTR_pairs.jpg|thumb|left|400 px]]<br> | [[Image:S14_research_disTR_pairs.jpg|thumb|left|400 px]] | ||
<br style="clear:both;"/> | |||
==W/F pairings== | ==W/F pairings== | ||
[[Image:S14_M3D6-pairings.png|thumb|left|400px]] | [[Image:S14_M3D6-pairings.png|thumb|left|400px]] | ||
<br style="clear:both;"/> | |||
==ELISA data== | |||
===T/R=== | |||
'''Green/Blue''' <br> | |||
[[Media:S14_M3D6-TR-Grn-Blu-CNI.txt]] <br> | |||
[[Media:S14_M3D6-TR-Grn-Blu-CNII.txt]] <br> | |||
===W/F=== | |||
==qPCR data== | |||
===Files with CP values and additional notes=== | |||
The Excel files below contains all of the section-wide data. Two different CP values are listed per sample. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. | |||
[[Media:20109-S14_TR_qPCR.xlsx]]<br> | |||
[[Media:20109-S14_WF_qPCR.xlsx]] | |||
Note that the WF file contains two spreadsheets, one for the original in-class run, and one for the additional run for Yellow (A) and Green (B) groups. <font color=FF33FF>'''Note that everyone can learn something about cross-plate replication''' from Yellow team's data (C in original run) if they wish to.</font color> | |||
==qPCR section-wide curves== | |||
===T/R=== | |||
[[Image:S14_M3D5_TR_raw.jpg|thumb|400px|left|T/R section, Raw amplification curves]] | |||
[[Image:S14_M3D5_TR_noiseband.jpg|thumb|400px|center|T/R section, Log-scale amplification curves with noise threshold]] | |||
[[Image:S14_M3D5_TR_18S-melt.jpg|thumb|300px|right|T/R section, Melt curves for 18S samples]] | |||
[[Image:S14_M3D5_TR_CNII-melt.jpg|thumb|300px|left|T/R section, Melt curves for CN II samples]] | |||
[[Image:S14_M3D5_TR_CNI-melt.jpg|thumb|300px|center|T/R section, Melt curves for CN I samples]] | |||
[[Image:S14_M3D5_TR_18S-noiseband.jpg|thumb|300px|right|T/R section, Amp curves for 18S samples]] | |||
[[Image:S14_M3D5_TR_CNII-noiseband.jpg|thumb|300px|left|T/R section, Amp curves for CN II samples]] | |||
[[Image:S14_M3D5_TR_CNI-noiseband.jpg|thumb|300px|center|T/R section, Amp curves for CN I samples]] | |||
<br style="clear:both;"/> | |||
===W/F=== | |||
====Original run==== | |||
[[Image:S14_M3D5_WF_raw.jpg|thumb|400px|left|W/F section, Raw amplification curves]] | |||
[[Image:S14_M3D5_WF_noiseband.jpg|thumb|400px|center|W/F section, Log-scale amplification curves with noise threshold]] | |||
[[Image:S14_M3D5_WF_18S-melt.jpg|thumb|300px|right|W/F section, Melt curves for 18 samples]] | |||
[[Image:S14_M3D5_WF_CNII-melt.jpg|thumb|300px|left|W/F section, Melt curves for CN II samples]] | |||
[[Image:S14_M3D5_WF_CNI-melt.jpg|thumb|300px|center|W/F section, Melt curves for CN I samples]] | |||
[[Image:S14_M3D5_WF_18S-noiseband.jpg|thumb|300px|right|W/F section, Amp curves for 18S samples]] | |||
[[Image:S14_M3D5_WF_CNII-noiseband.jpg|thumb|300px|left|W/F section, Amp curves for CN II samples]] | |||
[[Image:S14_M3D5_WF_CNI-noiseband.jpg|thumb|300px|center|W/F section, Amp curves for CN I samples]] | |||
<br style="clear:both;"/> | |||
====Extra run for Ylw and Grn groups==== | |||
[[Image:S14_M3D5_WFxt_raw.jpg|thumb|400px|left|W/F section, Raw amplification curves]] | |||
[[Image:S14_M3D5_WFxt_noiseband.jpg|thumb|400px|center|W/F section, Log-scale amplification curves with noise threshold]] | |||
[[Image:S14_M3D5_WFxt_18S-melt.jpg|thumb|300px|right|W/F section, Melt curves for 18 samples]] | |||
[[Image:S14_M3D5_WFxt_CNII-melt.jpg|thumb|300px|left|W/F section, Melt curves for CN II samples]] | |||
[[Image:S14_M3D5_WFxt_CNI-melt.jpg|thumb|300px|center|W/F section, Melt curves for CN I samples]] | |||
[[Image:S14_M3D5_WFxt_18S-noiseband.jpg|thumb|300px|right|W/F section, Amp curves for 18S samples]] | |||
[[Image:S14_M3D5_WFxt_CNII-noiseband.jpg|thumb|300px|left|W/F section, Amp curves for CN II samples]] | |||
[[Image:S14_M3D5_WFxt_CNI-noiseband.jpg|thumb|300px|center|W/F section, Amp curves for CN I samples]] | |||
Latest revision as of 12:01, 7 May 2014
W/F PG
Media:S14_M3D6-WF-PG-Red-Org.txt
Media:S14_M3D6-WF-PG-Ylw-Grn.txt
T/R pairings
W/F pairings
ELISA data
T/R
Green/Blue
Media:S14_M3D6-TR-Grn-Blu-CNI.txt
Media:S14_M3D6-TR-Grn-Blu-CNII.txt
W/F
qPCR data
Files with CP values and additional notes
The Excel files below contains all of the section-wide data. Two different CP values are listed per sample. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set.
Media:20109-S14_TR_qPCR.xlsx
Media:20109-S14_WF_qPCR.xlsx
Note that the WF file contains two spreadsheets, one for the original in-class run, and one for the additional run for Yellow (A) and Green (B) groups. Note that everyone can learn something about cross-plate replication from Yellow team's data (C in original run) if they wish to.
qPCR section-wide curves
T/R
W/F
Original run
Extra run for Ylw and Grn groups
