Talk:20.109(S14):Transcript-level analysis (Day6): Difference between revisions
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===W/F=== | ===W/F=== | ||
====Original run==== | |||
[[Image:S14_M3D5_WF_raw.jpg|thumb|400px|left|W/F section, Raw amplification curves]] | [[Image:S14_M3D5_WF_raw.jpg|thumb|400px|left|W/F section, Raw amplification curves]] | ||
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====Extra run for Ylw and Grn groups==== | |||
[[Image:S14_M3D5_WFxt_raw.jpg|thumb|400px|left|W/F section, Raw amplification curves]] | |||
[[Image:S14_M3D5_WFxt_noiseband.jpg|thumb|400px|center|W/F section, Log-scale amplification curves with noise threshold]] | |||
[[Image:S14_M3D5_WFxt_18S-melt.jpg|thumb|300px|right|W/F section, Melt curves for 18 samples]] | |||
[[Image:S14_M3D5_WFxt_CNII-melt.jpg|thumb|300px|left|W/F section, Melt curves for CN II samples]] | |||
[[Image:S14_M3D5_WFxt_CNI-melt.jpg|thumb|300px|center|W/F section, Melt curves for CN II samples]] | |||
[[Image:S14_M3D5_WFxt_18S-noiseband.jpg|thumb|300px|right|W/F section, Amp curves for 18S samples]] | |||
[[Image:S14_M3D5_WFxt_CNII-noiseband.jpg|thumb|300px|left|W/F section, Amp curves for CN II samples]] | |||
[[Image:S14_M3D5_WFxt_CNI-noiseband.jpg|thumb|300px|center|W/F section, Amp curves for CN II samples]] |
Revision as of 11:12, 6 May 2014
T/R pairings
W/F pairings
qPCR data
Files with CP values and additional notes
The Excel files below contains all of the section-wide data. Two different CP values are listed per sample. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set.
Media:20109-S14_TR_qPCR.xlsx
Media:20109-S14_WF_qPCR.xlsx
qPCR section-wide curves
T/R
W/F
Original run