Talk:20.109(S14):Transcript-level analysis (Day6): Difference between revisions

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===W/F===
===W/F===
====Original run====


[[Image:S14_M3D5_WF_raw.jpg|thumb|400px|left|W/F section, Raw amplification curves]]
[[Image:S14_M3D5_WF_raw.jpg|thumb|400px|left|W/F section, Raw amplification curves]]
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<br style="clear:both;"/>
<br style="clear:both;"/>
====Extra run for Ylw and Grn groups====
[[Image:S14_M3D5_WFxt_raw.jpg|thumb|400px|left|W/F section, Raw amplification curves]]
[[Image:S14_M3D5_WFxt_noiseband.jpg|thumb|400px|center|W/F section, Log-scale amplification curves with noise threshold]]
[[Image:S14_M3D5_WFxt_18S-melt.jpg|thumb|300px|right|W/F section, Melt curves for 18 samples]]
[[Image:S14_M3D5_WFxt_CNII-melt.jpg|thumb|300px|left|W/F section, Melt curves for CN II samples]]
[[Image:S14_M3D5_WFxt_CNI-melt.jpg|thumb|300px|center|W/F section, Melt curves for CN II samples]]
[[Image:S14_M3D5_WFxt_18S-noiseband.jpg|thumb|300px|right|W/F section, Amp curves for 18S samples]]
[[Image:S14_M3D5_WFxt_CNII-noiseband.jpg|thumb|300px|left|W/F section, Amp curves for CN II samples]]
[[Image:S14_M3D5_WFxt_CNI-noiseband.jpg|thumb|300px|center|W/F section, Amp curves for CN II samples]]

Revision as of 11:12, 6 May 2014

T/R pairings


W/F pairings


qPCR data

Files with CP values and additional notes

The Excel files below contains all of the section-wide data. Two different CP values are listed per sample. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set.

Media:20109-S14_TR_qPCR.xlsx
Media:20109-S14_WF_qPCR.xlsx

qPCR section-wide curves

T/R

T/R section, Raw amplification curves
T/R section, Log-scale amplification curves with noise threshold
T/R section, Melt curves for 18S samples
T/R section, Melt curves for CN II samples
T/R section, Melt curves for CN II samples
T/R section, Amp curves for 18S samples
T/R section, Amp curves for CN II samples
T/R section, Amp curves for CN II samples


W/F

Original run

W/F section, Raw amplification curves
W/F section, Log-scale amplification curves with noise threshold
W/F section, Melt curves for 18 samples
W/F section, Melt curves for CN II samples
W/F section, Melt curves for CN II samples
W/F section, Amp curves for 18S samples
W/F section, Amp curves for CN II samples
W/F section, Amp curves for CN II samples


Extra run for Ylw and Grn groups

W/F section, Raw amplification curves
W/F section, Log-scale amplification curves with noise threshold
W/F section, Melt curves for 18 samples
W/F section, Melt curves for CN II samples
W/F section, Melt curves for CN II samples
W/F section, Amp curves for 18S samples
W/F section, Amp curves for CN II samples
W/F section, Amp curves for CN II samples