Talk:20.109(S14):Diagnostic primer design (Day2)

T/R Section

Sensitivity

Team color	Forward (F) primer	Reverse (R) primer	Target site (e.g. bp 60), F	Target side (e.g., bp 300), R	Is bp # for VC or EH? Which gene?
Red	CAC CAG GTT GAT ACT GCC TGA C	CCT ATC CCG AAC CAA ACC CTG	bp 1	bp 260	EH, SSU rRNA
Green
Pink	ACC AGG TTG ATT CTG CCT GAC	TCT CTC ACG CTC CCT CTC C	bp1/bp2	bp252/bp290	EH/VC SSU rRna

Specificity

Team Color	Forward primer	Reverse primer	Target site (e.g. bp 60), F	Target side (e.g., bp 300), R	Is bp # for VC or EH? Which gene?
Yellow
Blue	GATTCTGCCTGACGTAGATGC	CTGCTGGCACCAAACTTGC	10 bp	408 bp	E. hellem (AF118143)
Orange			Silver