Talk:20.109(S14):Diagnostic primer design (Day2): Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 139: | Line 139: | ||
| | | | ||
|- | |- | ||
|Blue | |||
| CAC CAG GTT GAT TCT GCC TGA C | |||
| CTG CTG GCA CCA GAC TTG C | |||
| | |||
| | |||
|EH | |||
|Silver | |Silver | ||
| CAC CAG GTT GAT TCT GCC TGA C | | CAC CAG GTT GAT TCT GCC TGA C | ||
Line 161: | Line 167: | ||
|- | |- | ||
|} | |} | ||
<br style="clear:both;"/> | <br style="clear:both;"/> |
Revision as of 09:34, 13 February 2014
T/R Section
Sensitivity
Team color | Forward (F) primer | Reverse (R) primer | Target site (e.g. bp 60), F | Target side (e.g., bp 300), R | Is bp # for VC or EH? Which gene? |
Red | CAC CAG GTT GAT ACT GCC TGA C | CCT ATC CCG AAC CAA ACC CTG | bp 1 | bp 260 | EH, SSU rRNA |
Green | |||||
Pink | ACC AGG TTG ATT CTG CCT GAC | TCT CTC ACG CTC CCT CTC C | bp1/bp2 | bp252/bp290 | EH/VC SSU rRna |
Specificity
Team Color | Forward primer | Reverse primer | Target site (e.g. bp 60), F | Target side (e.g., bp 300), R | Is bp # for VC or EH? Which gene? |
Yellow | |||||
Blue | GATTCTGCCTGACGTAGATGC | CTGCTGGCACCAAACTTGC | 10 bp | 408 bp | E. hellem (AF118143) |
Orange |
W/F Section
Sensitivity
Team color | Forward (F) primer | Reverse (R) primer | Target site (e.g. bp 60), F | Target site (e.g., bp 300), R | Is bp # for VC or EH? Which gene? |
Pink | ACC AGG TTG ATT CTG CTG ACG | TTGCGAGCATACTATCCCCAG | bp 1/2 | bp 741/768 | VC/EH SSU rRNA |
Red | ACC AGG TTG ATT CTG CCT GAC | ATA TTA CCG CGG CTG CTG | bp 1/2 | bp 380/421 | VC/EH small subunit rRNA |
Orange | CCA GGT TGA CTC TGC CTG ACG TCG | ||||
Yellow | CAC CAG GTT GAT TCT GCC TGA C | CCA TCC ATT TCC AAC GAC CAT G | 1 | 947 | EH |
Specificity
Team color | Forward (F) primer | Reverse (R) primer | Target site (e.g. bp 60), F | Target site (e.g., bp 300), R | Is bp # for VC or EH? Which gene? | ||||||
White | |||||||||||
Blue | CAC CAG GTT GAT TCT GCC TGA C | CTG CTG GCA CCA GAC TTG C | EH | Silver | CAC CAG GTT GAT TCT GCC TGA C | TTC ACC ATA GAC ACT ACT GGA GC | 1 | 441 | EH | ||
Purple | GTCTGGCTGTGTAGATAACTACG | CACTGTGAGACCTCGCATC | 102 | 465 | VC | ||||||
Green | agattgctcccacgtccaag | caacgaccatgcaccactc | 251 | 872 | VC |