Talk:20.109(S13):Transcript-level analysis (Day6): Difference between revisions

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(New page: ==qPCR data== ===T/R=== The text file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. T...)
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Revision as of 08:58, 3 May 2013

qPCR data

T/R

The text file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. (T/R Purple, because your samples ran separately, there were not enough of them to make a per RNA threshold reasonable.)

Media:20109-S13_TR_qPCR.txt

W/F

The text file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set.

Media:20109-S13_WF_qPCR.txt

qPCR section-wide curves

T/R section, Raw amplification curves
T/R section, Log-scale amplification curves with noise threshold
T/R section, Melt curves for 18 samples
T/R section, Melt curves for CN II samples
T/R section, Melt curves for CN II samples



W/F section, Raw amplification curves
W/F section, Log-scale amplification curves with noise threshold
W/F section, Melt curves for 18 samples
W/F section, Melt curves for CN II samples
W/F section, Melt curves for CN II samples

Cross-group meetings

Scheme

Red 1/Yellow 1

Red 2/Blue 1

Orange 1/Pink 2

Orange 2/Blue 2

Yellow 2/Green 2

Green 1/Purple 2

Pink 1/Purple 1

T/R