Talk:20.109(S13):Transcript-level analysis (Day6)

qPCR data

T/R

The Excel file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. (T/R Purple, because your samples ran separately, there were not enough of them to make a per RNA threshold reasonable.)

Media:20109-S13_TR_qPCR.xlsx

W/F

The Excel file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set.

Media:20109-S13_WF_qPCR.xlsx

qPCR section-wide curves

T/R

T/R section, Raw amplification curves

T/R section, Log-scale amplification curves with noise threshold

T/R section, Melt curves for 18S samples

T/R section, Melt curves for CN II samples

T/R section, Melt curves for CN II samples

T/R section, Amp curves for 18S samples

T/R section, Amp curves for CN II samples

T/R section, Amp curves for CN II samples

W/F

W/F section, Raw amplification curves

W/F section, Log-scale amplification curves with noise threshold

W/F section, Melt curves for 18 samples

W/F section, Melt curves for CN II samples

W/F section, Melt curves for CN II samples

Cross-group meetings

T/R Scheme

Red 1/Yellow 1: Marisol/Dominique

Red 2/Blue 1: Nick/Justin

Orange 1/Platinum 2: Daphne/Katie

Platinum 1/Blue 2: Emily/Noa

Yellow 2/Green 2: Ebaa/Jesse

Green 1/Purple 1:Nikki/Sarah

Orange 2/Purple 2: Stas/Mitali

W/F Scheme

Blue 1/Platinum 2

• Emmanuel/Shanthi

Blue 2/Green 2

• Divya/Steve

Green 1/Platinum 1

• Kevin/Eric

Yellow 1/Orange 2

• Karinna/Katie

Pink 2/Purple 1

• Alice/Zeinab

Purple 2/Orange 1

• Jenny V./Ameila

Yellow 2/Pink 1

• Jenny W./Helen