Talk:20.109(S13):Transcript-level analysis (Day6): Difference between revisions
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===T/R=== | ===T/R=== | ||
The | The Excel file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. (T/R Purple, because your samples ran separately, there were not enough of them to make a per RNA threshold reasonable.) | ||
[[Media:20109-S13_TR_qPCR. | [[Media:20109-S13_TR_qPCR.xlsx]] | ||
===W/F=== | ===W/F=== | ||
The | The Excel file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. | ||
[[Media:20109-S13_WF_qPCR. | [[Media:20109-S13_WF_qPCR.xlsx]] | ||
==qPCR section-wide curves== | ==qPCR section-wide curves== |
Revision as of 12:22, 6 May 2013
qPCR data
T/R
The Excel file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. (T/R Purple, because your samples ran separately, there were not enough of them to make a per RNA threshold reasonable.)
W/F
The Excel file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set.
qPCR section-wide curves
Cross-group meetings
T/R Scheme
Red 1/Yellow 1: Marisol/Dominique
Red 2/Blue 1: Nick/Justin
Orange 1/Platinum 2: Daphne/Katie
Platinum 1/Blue 2: Emily/Noa
Yellow 2/Green 2: Ebaa/Jesse
Green 1/Purple 1:Nikki/Sarah
Orange 2/Purple 2: Stas/Mitali