Talk:20.109(S13):Induce protein and evaluate DNA (Day5)
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Induced pellet example
TR O/N Cultures and Gels
Below you should list the OD600 values of the induced cultures (stock value), as well as your pellet observations. (Is the color dull or bright? More yellow or more green?) The teaching faculty will fill in this information for the cultures needing to be grown overnight.
Group | OD WT | OD D24H/E67K/T79P/M124S | OD Chosen mutant | Pellets yellow? |
---|---|---|---|---|
Example | 0.95 | 1.2 (D24H) | 0.82 (G20P) | Y, bright ylw-grn |
Red | 0.22 | 0.51 (D24H) | 0.55 | grn, grn, ylw (+hint of grn) |
Orange | 0.36 | 0.36 (D24H) | 0.38 | grn, grn, ylw (+hint of grn) |
Yellow | 0.39 | 2.12 (T79P) | 3.36 (K77E2--not the chosen mutant) extra is 0.17 |
bright yellow; ylw (+hint of grn) |
Green | 0.34 | 1.59 (T79P) | 1.16 (M71R) | Bright Yellow |
Blue | 0.035 | 0.036 (M124S) | 0.088 (M71S) | Y, bright ylw |
Purple | 0.30 | 0.58 (M124S) | 0.32 | ylw-grn, ylw-grn, grn |
Platinum | 0.52 | 0.75 (M124S) | 0.38 | ylw-grn, ylw-grn, grn |
Gels
WF O/N Cultures and Gels
Below you should list the OD600 values of the induced cultures (stock value), as well as your pellet observations. (Is the color dull or bright? More yellow or more green?) The teaching faculty will fill in this information for the cultures needing to be grown overnight.
Group | OD WT | OD D24H/E67K/T79P/M124S | OD mutant option 1 | OD mutant option 2 | Pellets yellow? |
---|---|---|---|---|---|
Example | 0.95 | 1.2 (D24H) | 0.82 (G20P) | N/A | Y, bright ylw-grn |
Red | |||||
Orange | |||||
Yellow | |||||
Green | |||||
Blue | |||||
Pink | |||||
Purple | |||||
Platinum |
Gels