Talk:20.109(S13):Induce protein and evaluate DNA (Day5): Difference between revisions
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|- | |- | ||
|Yellow | |Yellow | ||
| | |0.39 | ||
| | |2.12 (T79P) | ||
| | |3.36 (K77E2--not the chosen mutant) | ||
| | |bright yellow | ||
|- | |- | ||
|Green | |Green |
Revision as of 14:56, 2 April 2013
Induced pellet example
TR O/N Cultures and Gels
Below you should list the OD600 values of the induced cultures (stock value), as well as your pellet observations. (Is the color dull or bright? More yellow or more green?) The teaching faculty will fill in this information for the cultures needing to be grown overnight.
Group | OD WT | OD D24H/E67K/T79P/M124S | OD Chosen mutant | Pellets yellow? |
---|---|---|---|---|
Example | 0.95 | 1.2 (D24H) | 0.82 (G20P) | Y, bright ylw-grn |
Red | ||||
Orange | ||||
Yellow | 0.39 | 2.12 (T79P) | 3.36 (K77E2--not the chosen mutant) | bright yellow |
Green | 0.34 | 1.59 (T79P) | 1.16 (M71R) | Bright Yellow |
Blue | 0.035 | 0.036 (M124S) | 0.088 (M71S) | Y, bright ylw |
Pink | ||||
Purple | ||||
Platinum |
Gels
WF O/N Cultures and Gels
Below you should list the OD600 values of the induced cultures (stock value), as well as your pellet observations. (Is the color dull or bright? More yellow or more green?) The teaching faculty will fill in this information for the cultures needing to be grown overnight.
Group | OD WT | OD D24H/E67K/T79P/M124S | OD Chosen mutant | Pellets yellow? |
---|---|---|---|---|
Example | 0.95 | 1.2 (D24H) | 0.82 (G20P) | Y, bright ylw-grn |
Red | ||||
Orange | ||||
Yellow | ||||
Green | ||||
Blue | ||||
Pink | ||||
Purple | ||||
Platinum |
Gels