Talk:20.109(S13):Context-setting and primer design (Day1): Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
| Line 4: | Line 4: | ||
When you have finished, post your primer sequences (from 5' to 3') so that they can be ordered. | When you have finished, post your primer sequences (from 5' to 3') so that they can be ordered. | ||
I will name your primers by the approximate base-pair that they target in ''V corneae'' or ''E hellem'', so please share this information as well. | |||
===Sensitivity=== | ===Sensitivity=== | ||
| Line 13: | Line 15: | ||
|Target site (e.g. bp 60), F | |Target site (e.g. bp 60), F | ||
|Target side (e.g., bp 300), R | |Target side (e.g., bp 300), R | ||
|Is bp # for VC or EH? | |Is bp # for VC or EH? Which gene? | ||
|- | |- | ||
| Grey (Platinum) :) | | Grey (Platinum) :) | ||
| Line 53: | Line 55: | ||
|Forward primer | |Forward primer | ||
|Reverse primer | |Reverse primer | ||
|Target site (e.g. bp 60), F | |||
|Target side (e.g., bp 300), R | |||
|Is bp # for VC or EH? Which gene? | |||
|- | |- | ||
| Purple | | Purple | ||
| | |||
| | |||
| | |||
| | | | ||
| | | | ||
|- | |- | ||
|Red | |Red | ||
| | |||
| | |||
| | |||
| | | | ||
| | | | ||
|- | |- | ||
|Orange | |Orange | ||
| | |||
| | |||
| | |||
| | | | ||
| | | | ||
|- | |- | ||
|Blue | |Blue | ||
| | |||
| | |||
| | |||
| | | | ||
| | | | ||
Revision as of 15:00, 7 February 2013
T/R section
Please sign up for one of the design challenges -- four teams per challenge -- right away.
When you have finished, post your primer sequences (from 5' to 3') so that they can be ordered.
I will name your primers by the approximate base-pair that they target in V corneae or E hellem, so please share this information as well.
Sensitivity
| Team color | Forward (F) primer | Reverse (R) primer | Target site (e.g. bp 60), F | Target side (e.g., bp 300), R | Is bp # for VC or EH? Which gene? |
| Grey (Platinum) :) | |||||
| Green | |||||
| Pink | |||||
| Yellow |
Specificity
| Team Color | Forward primer | Reverse primer | Target site (e.g. bp 60), F | Target side (e.g., bp 300), R | Is bp # for VC or EH? Which gene? |
| Purple | |||||
| Red | |||||
| Orange | |||||
| Blue |
W/F section
Please sign up for one of the design challenges -- four teams per challenge -- right away.
When you have finished, post your primer sequences (from 5' to 3') so that they can be ordered.
Sensitivity
| Team color | Forward primer | Reverse primer |
Specificity
| Team color | Forward primer | Reverse primer |
