Talk:20.109(S13):Characterize protein expression (Day7): Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→W/F) |
(→W/F) |
||
Line 29: | Line 29: | ||
[[Image:S13_M2D7_WF_Pink.JPG | center |thumb|250px | W/F Gel, Pink group]] | [[Image:S13_M2D7_WF_Pink.JPG | center |thumb|250px | W/F Gel, Pink group]] | ||
[[Image:S13_M2D7_WF_Purple.JPG | left |thumb|250px | W/F Gel, Purple group -- really Pink]] | [[Image:S13_M2D7_WF_Purple.JPG | left |thumb|250px | W/F Gel, Purple group -- really Pink]] | ||
[[Image:S13_M2D7_WF_Platinum.JPG | center |thumb|250px | W/F Gel, Platinum group]] | [[Image:S13_M2D7_WF_Platinum.JPG | center |thumb|250px | W/F Gel, Platinum group -- really Purple]] | ||
<br style="clear:both;"/> | <br style="clear:both;"/> |
Revision as of 14:30, 18 April 2013
SDS-PAGE analysis tips
You can look up the expected molecular weight of IPC using the sequence file (from Day 1 or Day 5) and this link or a similar website. Be sure to add ~ 3 KDa for the size of the N-terminus of pRSET (His tag, etc)!
If you see two strong bands on your gel, what do you think the second one is? As a starting hint, look back at the Day 6 protocol and carefully consider what is present in the unpurified protein tubes.
What other assays might you consider in relation to SDS-PAGE in order to take a holistic view of your data?
SDS-PAGE images
T/R
W/F