Talk:20.109(S13):Assess protein function (Day8): Difference between revisions
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| M124S: 0.4115; 3.9341 | | M124S: 0.4115; 3.9341 | ||
| D80R: 1.2991; 2.8465 | | D80R: 1.2991; 2.8465 | ||
| | | small difference between 0% and 100% for our mutant, affinity decrease and cooperativity increase | ||
|- | |- | ||
|Orange | |Orange |
Revision as of 12:58, 11 April 2013
Raw Data
Raw calcium titration data is posted below.
Analysis, T/R
Please be mindful of significant figures when you fill in this table. Do not state far more precise numbers than you can believe.
Group Colour | D24H, E67K, T79P, or M124S? | X#Z Mutant | Most believable KD; n for WT | Most believable KD; n for reference | Most believable KD; n for X#Z mutant | Comments (e.g., very low fluorescence) |
Plt | M124S | D80R | WT: 0.0976; 1.4751 | M124S: 0.4115; 3.9341 | D80R: 1.2991; 2.8465 | small difference between 0% and 100% for our mutant, affinity decrease and cooperativity increase |
Orange | D24H | G25Q | 0.1462, n = 0.8310 | 0.6759, n = 2.5734 | 0.7118; n = -0.2595 | Very low fluorescence; oscillating trend... |
Analysis, W/F
Analysis, T/R
Please be mindful of significant figures when you fill in this table. Do not state far more precise numbers than you can believe.
Group Colour | D24H, E67K, T79P, or M124S? | X#Z Mutant | Most believable KD; n for WT | Most believable KD; n for reference | Most believable KD; n for X#Z mutant | Comments (e.g., very low fluorescence) |