Talk:20.109(S12):Initiate cell culture (Day2): Difference between revisions
Haozhi Lin (talk | contribs) |
|||
| Line 61: | Line 61: | ||
==Designs (T/R)== | ==Designs (T/R)== | ||
Team Orange (with Team Yellow): Papers have shown that TGF Beta 1 is an important growth factor in maintaining a chondrogenic phenotype. Our experiment will examine if there is a dose dependency of TGF Beta 1 on chondrocyte dedifferentiation. We propose examine how well 0 ng/mL, 2ng/mL and 10ng/mL of TGF B-1 maintains chondrogenic phenotype using 3% low viscosity agarose, and 20x10^6 cells/mL. I think that the chondrocytes with no TGF B-1 will dedifferentiate, because they will be growth factor deficient. | Team Orange (with Team Yellow): Papers have shown that TGF Beta 1 is an important growth factor in maintaining a chondrogenic phenotype. Our experiment will examine if there is a dose dependency of TGF Beta 1 on chondrocyte dedifferentiation. We propose examine how well 0 ng/mL, 2ng/mL and 10ng/mL of TGF B-1 maintains chondrogenic phenotype using 3% low viscosity agarose, and 20x10^6 cells/mL. I think that the chondrocytes with no TGF B-1 will dedifferentiate, because they will be growth factor deficient. The 2ng samples I would expect to maintain their phenotype. If the 10ng/mL is not cytotoxic to the cells, then I also expect them to maintain their phenotype. | ||
Team Green: | Team Green: | ||
Revision as of 03:44, 19 April 2012
Plan for Day 2
Group 1 should arrive by 1:05 pm at the latest and immediately go to the tissue culture room. When everyone has arrived, we will thaw your cells in the water bath. After you have finished your culture preparations (ideally by 3 pm), you can take a 10 minute break to refresh your minds, and then will take a short quiz.
Group 2 should arrive by 2:45 pm at the latest and will begin by taking the quiz. If all goes well, you will begin working in the tissue culture room at 3 pm. If your culture preparations that involve a special physical set-up, you can come earlier and work in the extra tissue culture hood.
Note that you may be asked to switch your group number based on grouping together people working with the same type of cells, asking people who need special reagents/equipment to go in the second group, etc.
Notebooks may be handed in by the following Tuesday and Wednesday (when we don't have lab), 1 pm outside my office.
| Arrival time (at latest!) | 1:05 pm | 2:45 pm |
| Team colour 1 | Red | Pink |
| Team colour 2 | Blue | Yellow |
| Team colour 3 | Green | Orange (w/Team Yellow) |
| Team colour 4 | Purple |
W/F
| Arrival time (at latest!) | 1:05 pm | 2:45 pm |
| Team colour 1 | Pink | Blue |
| Team colour 2 | Purple | Yellow |
| Team colour 3 | Orange | Green |
| Team colour 4 | Red |
Designs (T/R)
Team Orange (with Team Yellow): Papers have shown that TGF Beta 1 is an important growth factor in maintaining a chondrogenic phenotype. Our experiment will examine if there is a dose dependency of TGF Beta 1 on chondrocyte dedifferentiation. We propose examine how well 0 ng/mL, 2ng/mL and 10ng/mL of TGF B-1 maintains chondrogenic phenotype using 3% low viscosity agarose, and 20x10^6 cells/mL. I think that the chondrocytes with no TGF B-1 will dedifferentiate, because they will be growth factor deficient. The 2ng samples I would expect to maintain their phenotype. If the 10ng/mL is not cytotoxic to the cells, then I also expect them to maintain their phenotype.
Team Green: Our experiment will examine the effects on mesenchymal cell differentiation based off of initial cell concentration.To examine this we will grow two cells cultures on medium viscosity,3 % agarose gel. One of the cultures will have initial concentration of 10^7 cells/mL, and the other of 10^8 cells/mL. We assume that the cells with initial concentration of 10^7 cells/mL will differentiate more effectively.
Team Blue: We will examine the effects of alginate gel viscosity on cell differentiation rate (and possibly cartilage structure formation). We will grow two cell cultures, 1 of low and 1 of high viscosity, in 2% alginate matrix, each at 10^7 cells/mL. We hypothesize that cells grown in the less viscous matrix will grow differentiate faster.
