Talk:20.109(S12):Induce protein and evaluate DNA (Day5): Difference between revisions
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[[Image:S12_M2D5_WF_gel-Yellow.jpg|thumb|center|400px|W/F Yellow]] | [[Image:S12_M2D5_WF_gel-Yellow.jpg|thumb|center|400px|W/F Yellow]] | ||
[[Image:S12_M2D5_WF_gel-Green.jpg|thumb|right|400px|W/F Green]] | [[Image:S12_M2D5_WF_gel-Green.jpg|thumb|right|400px|W/F Green]] | ||
[[Image:S12_M2D5_WF_gel-Blue.jpg|thumb|left|400px|W/F Blue]] | [[Image:S12_M2D5_WF_gel-Blue.jpg|thumb|left|400px|W/F Blue -- NOTE: Lanes 8 and 9 are switched]] | ||
[[Image:S12_M2D5_WF_gel-Pink.jpg|thumb|center|400px|W/F Pink]] | [[Image:S12_M2D5_WF_gel-Pink.jpg|thumb|center|400px|W/F Pink]] | ||
[[Image:S12_M2D5_WF_gel-Purple.jpg|thumb|left|400px|W/F Purple]] | [[Image:S12_M2D5_WF_gel-Purple.jpg|thumb|left|400px|W/F Purple]] | ||
Revision as of 13:00, 6 April 2012
Induced pellet example
TR O/N Cultures and Gels
Below you should list the OD600 values of the induced cultures (at 1:10 dilutions), as well as your pellet observations. The teaching faculty will fill in this information for the cultures needing to be grown overnight.
| Group | OD WT | OD M124S/E67K/T79P | OD Chosen mutant | Pellets yellow? |
|---|---|---|---|---|
| Red | ||||
| Orange | .067 | .024 | Mutant 1: .095 / Mutant 2: .065 | Yes |
| Yellow | 0.069 | 0.138 | 0.062 | Yes |
| Green | ||||
| Blue | ||||
| Pink | 0.071 | 0.317 | 0.119 | Y: bright yellow green WT, F65D; dull/pale yellow T79P |
| Purple |
Gels
WF O/N Cultures and Gels
Below you should list the OD600 values of the induced cultures (at 1:10 dilutions), as well as your pellet observations. The teaching faculty will fill in this information for the cultures needing to be grown overnight.
| Group | OD WT | OD M124S/E67K/T79P | OD Chosen mutant | Pellets yellow? |
|---|---|---|---|---|
| Red | ||||
| Orange | ||||
| Yellow | ||||
| Green | ||||
| Blue | ||||
| Pink | ||||
| Purple |
Gels
