Talk:20.109(S11):Testing cell viability (Day3): Difference between revisions
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Testing chondrocytes with and without the presence of ascorbate (a growth factor). Beads a made from low viscosity alginate. | Testing chondrocytes with and without the presence of ascorbate (a growth factor). Beads a made from low viscosity alginate. | ||
[[Image:WithAscorbate.JPG|thumb|center|Live cells shown with ascorbate added]] | [[Image:WithAscorbate.JPG|thumb|center|Live cells shown with ascorbate added]] | ||
[[Image:WithoutAscorbate.JPG|thumb|center|Few cells can be seen in the sample without ascorbate, most likely due to cells falling out of the bead.]] | |||
|- | |- | ||
|4:10-4:35 | |4:10-4:35 |
Revision as of 17:12, 21 April 2011
T/R schedule
Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.
Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.
Microscope time | Arrival time (at latest!) | Team colour |
2:05-2:30 | 1:05 | Yellow |
2:30-2:55 | 1:20 | Red |
2:55-3:20 | 1:45 | Blue
mesenchymal stem cells grown in low viscosity alginate with various conditions |
3:20-3:45 | 2:10 | Purple
mesenchymal stem cells grown in low viscosity alginate with various conditions |
3:45-4:10 | 2:35 | Pink
Testing chondrocytes with and without the presence of ascorbate (a growth factor). Beads a made from low viscosity alginate. |
4:10-4:35 | 3:00 | Orange |
4:35-5:00 | 3:25 | Green |
W/F schedule
Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.
Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.
Microscope time | Arrival time (at latest!) | Team colour |
2:05-2:30 | 1:05 | Purple |
2:30-2:55 | 1:20 | Pink |
2:55-3:20 | 1:45 | Blue |
3:20-3:45 | 2:10 | Green |
3:45-4:10 | 2:35 | Orange |
4:10-4:35 | 3:00 | Yellow |
4:35-5:00 | 3:25 | Red |