Talk:20.109(S11):Purify RNA and run affinity column (Day4): Difference between revisions
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(New page: ==Sign-up for column protocols== ===T/R=== You and your partner should propose a protocol in the table below. If you make changes to the 8-12 % due to not having enough RNA on Day 4, p...) |
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|1 | |1 | ||
|Red | |Red | ||
| | |.5 | ||
| | |4 | ||
| | |24 | ||
| | |Starting with a lower 8-12 concentration. | ||
|- | |- | ||
|2 | |2 | ||
|Orange | |Orange | ||
| | |50 | ||
| | |8 | ||
| | |16 | ||
| | |Repeat high-percentage run because the decrease in enrichment with increasing number of washes intrigued us | ||
|- | |- | ||
|3 | |3 | ||
|Yellow | |Yellow | ||
| | |1 | ||
| | |4 | ||
| | |24 | ||
| | |starting with a lower 8-12 concentration | ||
|- | |- | ||
|4 | |4 | ||
|Green | |Green | ||
| | |50 | ||
| | |4 | ||
| | |24 | ||
| | |Repeating high-percentage run for statistical reliability. | ||
|- | |- | ||
|5 | |5 | ||
|Blue | |Blue | ||
| | |2 | ||
| | |4 | ||
| | |32 | ||
| | |Check to see at what limit does the trend of more washes contribute to the yield hold true. | ||
|- | |- | ||
|6 | |6 | ||
|Pink | |Pink | ||
| | |2 | ||
| | |4 | ||
| | |24 | ||
| | |Repeat from last year's experiment | ||
|- | |- | ||
|7 | |7 | ||
|Purple | |Purple | ||
| | |10 | ||
| | |8 | ||
| | |32 | ||
| | |Interested in seeing the effect of introducing greater number of washes | ||
|} | |} | ||
</center> | </center> | ||
| Line 77: | Line 77: | ||
===W/F=== | ===W/F=== | ||
You and your partner should | You and your partner should propose a protocol in the table below. | ||
If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column. | If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column. | ||
<center> | |||
{| border="1" | |||
! Protocol# | |||
! Group | |||
! 8-12 % | |||
! Wash # (partner 1) | |||
! Wash # (partner 2) | |||
! Note changes to plan here | |||
|- | |||
|0 | |||
|Sample | |||
|5 | |||
|5 | |||
|15 | |||
|Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12. | |||
|- | |||
|1 | |||
|Red | |||
|2 | |||
|4 | |||
|24 | |||
|Repeat from last year's experiment | |||
|- | |||
|2 | |||
|Orange | |||
|2 | |||
|2 | |||
|48 | |||
|New experiment: Testing extremes of washing. | |||
|- | |||
|3 | |||
|Yellow | |||
|50 | |||
|8 | |||
|16 | |||
|Last year's experimenters observed a decrease in yield after more washes. Repeating to see if the break from the general trend is due to the conditions selected or experimenter variation. | |||
|- | |||
|4 | |||
|Green | |||
|.25 | |||
|4 | |||
|24 | |||
|New experiment, .25% pre-selection 8-12 to test lower limits of 8-12 recovery. | |||
|- | |||
|5 | |||
|Blue | |||
|10 | |||
|2 | |||
|34 | |||
|New experiment. Using 10% (we would have chosen 2%, but we suspect there could be contamination in our sample) but with extremes of washing. | |||
|- | |||
|6 | |||
|Pink | |||
|10 | |||
|8 | |||
|16 | |||
|Repeat from last year's | |||
|- | |||
|7 | |||
|Purple | |||
|50 | |||
|4 | |||
|24 | |||
|repeating last year's experiment that did not actually have 50% | |||
|} | |||
</center> | |||
<br style="clear:both;"/> | |||
Latest revision as of 08:10, 15 February 2011
Sign-up for column protocols
T/R
You and your partner should propose a protocol in the table below.
If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.
| Protocol# | Group | 8-12 % | Wash # (partner 1) | Wash # (partner 2) | Note changes to plan here |
|---|---|---|---|---|---|
| 0 | Sample | 5 | 5 | 15 | Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12. |
| 1 | Red | .5 | 4 | 24 | Starting with a lower 8-12 concentration. |
| 2 | Orange | 50 | 8 | 16 | Repeat high-percentage run because the decrease in enrichment with increasing number of washes intrigued us |
| 3 | Yellow | 1 | 4 | 24 | starting with a lower 8-12 concentration |
| 4 | Green | 50 | 4 | 24 | Repeating high-percentage run for statistical reliability. |
| 5 | Blue | 2 | 4 | 32 | Check to see at what limit does the trend of more washes contribute to the yield hold true. |
| 6 | Pink | 2 | 4 | 24 | Repeat from last year's experiment |
| 7 | Purple | 10 | 8 | 32 | Interested in seeing the effect of introducing greater number of washes |
W/F
You and your partner should propose a protocol in the table below.
If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.
| Protocol# | Group | 8-12 % | Wash # (partner 1) | Wash # (partner 2) | Note changes to plan here |
|---|---|---|---|---|---|
| 0 | Sample | 5 | 5 | 15 | Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12. |
| 1 | Red | 2 | 4 | 24 | Repeat from last year's experiment |
| 2 | Orange | 2 | 2 | 48 | New experiment: Testing extremes of washing. |
| 3 | Yellow | 50 | 8 | 16 | Last year's experimenters observed a decrease in yield after more washes. Repeating to see if the break from the general trend is due to the conditions selected or experimenter variation. |
| 4 | Green | .25 | 4 | 24 | New experiment, .25% pre-selection 8-12 to test lower limits of 8-12 recovery. |
| 5 | Blue | 10 | 2 | 34 | New experiment. Using 10% (we would have chosen 2%, but we suspect there could be contamination in our sample) but with extremes of washing. |
| 6 | Pink | 10 | 8 | 16 | Repeat from last year's |
| 7 | Purple | 50 | 4 | 24 | repeating last year's experiment that did not actually have 50% |
