Talk:20.109(S11):Purify RNA and run affinity column (Day4): Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→T/R) |
(→T/R) |
||
| Line 39: | Line 39: | ||
|3 | |3 | ||
|Yellow | |Yellow | ||
| | |1 | ||
| | |4 | ||
| | |24 | ||
| | |starting with a lower 8-12 concentration | ||
|- | |- | ||
|4 | |4 | ||
Revision as of 12:40, 10 February 2011
Sign-up for column protocols
T/R
You and your partner should propose a protocol in the table below.
If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.
| Protocol# | Group | 8-12 % | Wash # (partner 1) | Wash # (partner 2) | Note changes to plan here |
|---|---|---|---|---|---|
| 0 | Sample | 5 | 5 | 15 | Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12. |
| 1 | Red | .5 | 4 | 24 | Starting with a lower 8-12 concentration. |
| 2 | Orange | ||||
| 3 | Yellow | 1 | 4 | 24 | starting with a lower 8-12 concentration |
| 4 | Green | 50 | 4 | 24 | Repeating high-percentage run for statistical reliability. |
| 5 | Blue | ||||
| 6 | Pink | 2 | 4 | 24 | Repeat from last year's experiment |
| 7 | Purple |
W/F
You and your partner should propose a protocol in the table below.
If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.
| Protocol# | Group | 8-12 % | Wash # (partner 1) | Wash # (partner 2) | Note changes to plan here |
|---|---|---|---|---|---|
| 0 | Sample | 5 | 5 | 15 | Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12. |
| 1 | Red | ||||
| 2 | Orange | ||||
| 3 | Yellow | ||||
| 4 | Green | ||||
| 5 | Blue | ||||
| 6 | Pink | ||||
| 7 | Purple |
