Talk:20.109(S11):Complete DNA design (Day2): Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Huayu Ding (talk | contribs) (→T/R) |
(→T/R) |
||
| Line 15: | Line 15: | ||
| | | | ||
|- | |- | ||
| | |Pink | ||
| | |5'CCCGGGTAAGCACCTGTAGGATCGTACAGGTTTACGCAAGAAAATGGTTTGTTATAGTCGAATACCTCTGGCGGTGATATAACACCGTGCGTGTTGACTATTTTACCTCTGGCGGTGATAGGATCC3' <br> | ||
| | 5'AAATCCTATCACCGCCAGAGGTAAAATAGTCAACACGCACGGTGTTATATCACCGCCAGAGGTATTCGACTATAACAAACCATTTTCTTGCGTAAACCTGTACGATCCTACAGGTGCTTACCCGGG 3' | ||
|We are inserting a second copy of the Or1 sequence directly infront of Or2 to hopefully improve repressing of the operons | |||
|- | |- | ||
| | | | ||
Revision as of 12:24, 10 March 2011
High-level designs and implementation with primers
T/R
| Group | Forward primer; <br>Reverse primer | Summary of approach | |
|---|---|---|---|
| Blue | we are deleting part of the -35 region (after Or2) in the Pr promoter and adding 2 G's in the middle of -10 region (right after Or1). | ||
| Pink | 5'CCCGGGTAAGCACCTGTAGGATCGTACAGGTTTACGCAAGAAAATGGTTTGTTATAGTCGAATACCTCTGGCGGTGATATAACACCGTGCGTGTTGACTATTTTACCTCTGGCGGTGATAGGATCC3' 5'AAATCCTATCACCGCCAGAGGTAAAATAGTCAACACGCACGGTGTTATATCACCGCCAGAGGTATTCGACTATAACAAACCATTTTCTTGCGTAAACCTGTACGATCCTACAGGTGCTTACCCGGG 3' |
We are inserting a second copy of the Or1 sequence directly infront of Or2 to hopefully improve repressing of the operons | |
W/F
| Group | Forward primer; <br>Reverse primer | Summary of approach |
|---|---|---|
