Talk:20.109(S10):Testing cell viability (Day3): Difference between revisions
No edit summary |
No edit summary |
||
| Line 43: | Line 43: | ||
|- | |- | ||
|Red with Chelator | |Red with Chelator | ||
[[Image:DSCN0728.jpg| | [[Image:DSCN0728.jpg|450px]] | ||
|- | |- | ||
|Red without Chelator | |Red without Chelator | ||
| Line 49: | Line 49: | ||
|- | |- | ||
|Yellow with biological pH (7.2-7.4) | |Yellow with biological pH (7.2-7.4) | ||
[[Image:DSCN0729.JPG| | [[Image:DSCN0729.JPG|450px|caption]] | ||
|- | |- | ||
|This image was taken from the cross section of a bead at biological pH. | |This image was taken from the cross section of a bead at biological pH. | ||
| Line 57: | Line 57: | ||
|- | |- | ||
|Yellow with pH 8.0 | |Yellow with pH 8.0 | ||
[[Image:DSCN0733.JPG| | [[Image:DSCN0733.JPG|450px|caption]] | ||
|- | |- | ||
|This image was taken from the surface a bead at pH 8. The concentration of cells | |This image was taken from the surface a bead at pH 8. The concentration of cells | ||
| Line 66: | Line 66: | ||
5 x lower than in biological pH. | 5 x lower than in biological pH. | ||
|- | |- | ||
|Blue | |Blue | ||
|- | |||
|Orange | |Orange | ||
|- | |||
|Green | |Green | ||
|- | |||
|Purple | |Purple | ||
|- | |||
|Pink | |Pink | ||
|} | |} | ||
Revision as of 12:45, 22 April 2010
T/R schedule
Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.
Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.
| Microscope time | Arrival time (at latest!) | Team colour |
| 2:05-2:30 | 1:05 | Red |
| 2:30-2:55 | 1:20 | Yellow |
| 2:55-3:20 | 1:45 | Blue |
| 3:20-3:45 | 2:10 | Orange |
| 3:45-4:10 | 2:35 | Green |
| 4:10-4:35 | 3:00 | Purple |
| 4:35-5:00 | 3:25 | Pink |
| Team colour |
| Red with Chelator
|
| Red without Chelator
|
| Yellow with biological pH (7.2-7.4)
|
| This image was taken from the cross section of a bead at biological pH.
The concentration of cells as well as the ratio of dead (red) to live (green) cells is representative of all frames observed. The distribution of cells was uniform at different layers of the bead. |
| Yellow with pH 8.0
|
| This image was taken from the surface a bead at pH 8. The concentration of cells
as well as the ratio of dead (red) to live (green) cells is representative of all frames observed. The distribution of cells was uniform at different layers of the bead. There does not appear to be a significant difference in viability between high and low pH, but the density of cells in high pH was approximately 5 x lower than in biological pH. |
| Blue |
| Orange |
| Green |
| Purple |
| Pink |
| Start time | Team colour(s) |
| first (is the worst) | Yellow,Green,Red |
| second (is the best) | Purple,Orange, Blue |
| third (is ---) | Pink |
W/F schedule
Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.
Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.
| Microscope time | Arrival time (at latest!) | Team colour |
| 2:05-2:30 | 1:05 | Orange |
| 2:30-2:55 | 1:20 | Red |
| 2:55-3:20 | 1:45 | Yellow |
| 3:20-3:45 | 2:10 | Purple |
| 3:45-4:10 | 2:35 | Pink |
| 4:10-4:35 | 3:00 | Green |
| 4:35-5:00 | 3:25 | Blue |
[[[[]]]]
