Talk:20.109(S10):Start-up protein engineering (Day1): Difference between revisions
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| | |Mutation G21W and G23W | ||
| | |primer: C GAC AAG GAT TGG GAC TGG ACC ATC ACC ACA AAG GAA CTT GGC ACC G | ||
| | |Silent mutation changes a C to a T at position 302 in the pericam. (position 32 in calmodulin). | ||
|Enzyme KpnI | |||
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Revision as of 15:39, 9 March 2010
TR lab
| Team colour | Mutation X#Z | Primer (forward) | Silent mutation | New restriction site | ||||
| Teaching sample | S101L | GG AAC GGC TAC ATC CTC GCT GCG CAG TTA CGT CAC G | Ala 103 | FspI | ||||
| Red | D20R | GAA GCU UUC UCA UUA UUC CGU AAG GAU GGG GAC GGC ACC AUC | Ala15 | HindIII | ||||
| Orange | ||||||||
| Yellow | Mutation G21W and G23W | primer: C GAC AAG GAT TGG GAC TGG ACC ATC ACC ACA AAG GAA CTT GGC ACC G | Silent mutation changes a C to a T at position 302 in the pericam. (position 32 in calmodulin). | Enzyme KpnI | ||||
| Green | G23P | CA TTA TTC GAC AAG GAT CCG GAC GGC ACC ATC ACC ACA AAG G | none | BamHI | ||||
| Blue | D129P | GTT GAT GAA ATG ATA AGG GAA GCA CCU ATC GAT GGC GAT GGC C | Gly 410 | BtgZI | ||||
| Pink | G60P | 5' G CAG GAT ATG ATC AAT GAA GTC GAC GCT GAT CCC AAT GGA ACG 3’ | Asp 57 | Acc1 | ||||
| Purple | N137F | 5’ - GGT GAT GGC CAA GTA TTC TAT GAG GAG TTT G – 3' | Glu 139 | BseRI |
WF lab
| Team colour | Mutation X#Z | Primer (forward) | Silent mutation | New restriction site |
| Teaching sample | S101L | GG AAC GGC TAC ATC CTC GCT GCG CAG TTA CGT CAC G | Ala 103 | FspI |
| Red | ||||
| Orange | ||||
| Yellow | ||||
| Green | ||||
| Blue | ||||
| Pink | ||||
| Purple |
