Talk:20.109(S09): Luciferase assays and RNA prep (Day6): Difference between revisions

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Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (''Renilla''/firefly).
Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (''Renilla''/firefly).
Note: feel free to put ratios from either background-subtracted or raw values; they are likely to be quite similar. Hoewever, if you see this note, please mark which kind of data your ratios come from.


{| border="1"
{| border="1"
Line 10: Line 12:
! colspan="2" | scrambled
! colspan="2" | scrambled
! colspan="2" | experimental
! colspan="2" | experimental
! raw or subtracted?
|--
|--
|
|
Line 22: Line 25:
| Rep 1 ratio
| Rep 1 ratio
| Rep 2 ratio
| Rep 2 ratio
|
|--
|--
|Pink
|Pink
Line 34: Line 38:
|14.7
|14.7
|17.0
|17.0
|
|--
|--
|Green
|Green
Line 46: Line 51:
|2.8
|2.8
|2.6
|2.6
|Raw
|--
|--
|
|Orange
|
|.076
|
|22.3
|
|21.2
|
|29.0
|
|2.25
|
|1.74
|
|22.0
|
|22.8
|
|4.8
|4.1
|
|
|--
|--
|
|Yellow
|
|1.33
|
|.17
|
|20.8
|
|29.4
|
|1.42
|
|2.24
|
|25.74
|
|26.08
|
|1.17
|1.53
|
|
|--
|--
|
|Grey
|
|1.74
|
|1.10
|
|38.64
|
|34.59
|
|2.55
|
|2.90
|
|37.75
|
|35.83
|
|9.04
|
|8.80
|Raw
|--
|--
|
|Red
|
|2.02
|
|3.00
|
|21.36
|
|16.40
|
|1.31
|
|1.51
|
|20.78
|
|23.90
|
|1.53
|
|1.61
|Raw
|--
|--
|
|Blue
|
|1.57
|
|3.64
|
|14.78
|
|18.67
|
|1.75
|
|.51
|
|17.40
|
|15.16
|
|8.25
|6.87
|
|
|--
|--
|
|Purple
|
|1.03
|
|1.03
|
|40.4
|
|0.847* (not reliable)
|
|3.24
|
|3.90
|
|23.9
|
|29.8
|
|7.55
|
|8.33
|Raw
|--
|--
|}
|}
Line 124: Line 136:


Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (''Renilla''/firefly).
Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (''Renilla''/firefly).
Note: feel free to put ratios from either background-subtracted or raw values; they are likely to be quite similar. Hoewever, if you see this note, please mark which kind of data your ratios come from.


{| border="1"
{| border="1"
Line 132: Line 146:
! colspan="2" | scrambled
! colspan="2" | scrambled
! colspan="2" | experimental
! colspan="2" | experimental
! raw or subtracted?
|--
|--
|
|
Line 144: Line 159:
| Rep 1 ratio
| Rep 1 ratio
| Rep 2 ratio
| Rep 2 ratio
|
|--
|--
|Pink
|Pink
Line 156: Line 172:
|18.10
|18.10
|18.08
|18.08
|R A W
|--
|--
|Orange
|Orange
Line 168: Line 185:
|6.42
|6.42
|5.13
|5.13
|
|--
|--
|
|Red
|
|2.133
|
|2.6
|
|5.924
|
|14.4
|
|0.385
|
|1.636
|
|20.96
|
|19.89
|
|1.96
|1.72
|
|
|--
|--
|
|Blue
|
|1.019
|
|0.761658
|
|16.17009
|
|19.39064
|
|2.758433
|
|8.679711
|
|15.43884
|
|10.86473
|
|14.8144
|
|12.87329
|raw!
|--
|--
|
|Purple
|
|1.1016
|
|1.820
|
|32.05
|
|30.98
|
|1.369
|
|2.073
|
|35.384
|
|33.298
|
|1.189
|
|1.751
|raw
|--
|--
|
|Green
|
|0.878
|
|1.028
|
|31.548
|
|23.173
|
|1.562
|
|3.331
|
|24.934
|
|18.317
|
|4.434
|
|9.589
|Raw
|--
|--
|
|Yellow
|
|1.071
|
|0.942
|
|29.831
|
|29.021
|
|3.040
|
|3.575
|
|30.356
|
|31.690
|
|1.989
|
|2.203
|RAW
|--
|--
|
|Grey
|
|16.25
|
|6.42
|
|22.1
|
|18.6
|
|3.19
|
|4.02
|
|3.51
|
|6.14
|
|3.25
|8.35
|subtracted
|
|
|--
|--
|}
|}

Latest revision as of 11:20, 8 April 2009

T/R Luciferase Reactions

Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (Renilla/firefly).

Note: feel free to put ratios from either background-subtracted or raw values; they are likely to be quite similar. Hoewever, if you see this note, please mark which kind of data your ratios come from.

Team Colour none pLuc only validated scrambled experimental raw or subtracted?
Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio
Pink 4.5 4.0 20.7 19.1 2.73 1.92 18.9 22.9 14.7 17.0
Green .94 .97 36.4 26.3 2.7 2.4 27.6 29.1 2.8 2.6 Raw
Orange .076 22.3 21.2 29.0 2.25 1.74 22.0 22.8 4.8 4.1
Yellow 1.33 .17 20.8 29.4 1.42 2.24 25.74 26.08 1.17 1.53
Grey 1.74 1.10 38.64 34.59 2.55 2.90 37.75 35.83 9.04 8.80 Raw
Red 2.02 3.00 21.36 16.40 1.31 1.51 20.78 23.90 1.53 1.61 Raw
Blue 1.57 3.64 14.78 18.67 1.75 .51 17.40 15.16 8.25 6.87
Purple 1.03 1.03 40.4 0.847* (not reliable) 3.24 3.90 23.9 29.8 7.55 8.33 Raw

W/F Luciferase Reactions

Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (Renilla/firefly).

Note: feel free to put ratios from either background-subtracted or raw values; they are likely to be quite similar. Hoewever, if you see this note, please mark which kind of data your ratios come from.

Team Colour none pLuc only validated scrambled experimental raw or subtracted?
Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio
Pink 1.83 1.39 22.59 20.86 2.87 2.91 21.06 21.61 18.10 18.08 R A W
Orange 1.11 0.89 20.56 11.30 1.70 2.30 21.71 16.40 6.42 5.13
Red 2.133 2.6 5.924 14.4 0.385 1.636 20.96 19.89 1.96 1.72
Blue 1.019 0.761658 16.17009 19.39064 2.758433 8.679711 15.43884 10.86473 14.8144 12.87329 raw!
Purple 1.1016 1.820 32.05 30.98 1.369 2.073 35.384 33.298 1.189 1.751 raw
Green 0.878 1.028 31.548 23.173 1.562 3.331 24.934 18.317 4.434 9.589 Raw
Yellow 1.071 0.942 29.831 29.021 3.040 3.575 30.356 31.690 1.989 2.203 RAW
Grey 16.25 6.42 22.1 18.6 3.19 4.02 3.51 6.14 3.25 8.35 subtracted
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