Talk:20.109(S09): Luciferase assays and RNA prep (Day6): Difference between revisions
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Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (''Renilla''/firefly). | Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (''Renilla''/firefly). | ||
Note: feel free to put ratios from either background-subtracted or raw values; they are likely to be quite similar. Hoewver, if you see this note, please mark which kind of data your ratios come from. | |||
{| border="1" | {| border="1" | ||
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! colspan="2" | scrambled | ! colspan="2" | scrambled | ||
! colspan="2" | experimental | ! colspan="2" | experimental | ||
! subtracted or raw? | |||
|-- | |-- | ||
| | | | ||
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| Rep 1 ratio | | Rep 1 ratio | ||
| Rep 2 ratio | | Rep 2 ratio | ||
| | |||
|-- | |-- | ||
|Pink | |Pink | ||
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|14.7 | |14.7 | ||
|17.0 | |17.0 | ||
| | |||
|-- | |-- | ||
|Green | |Green | ||
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|2.8 | |2.8 | ||
|2.6 | |2.6 | ||
| | |||
|-- | |-- | ||
|Orange | |Orange | ||
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|4.8 | |4.8 | ||
|4.1 | |4.1 | ||
| | |||
|-- | |-- | ||
|Yellow | |Yellow | ||
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|1.17 | |1.17 | ||
|1.53 | |1.53 | ||
| | |||
|-- | |-- | ||
|Grey | |Grey | ||
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|9.04 | |9.04 | ||
|8.80 | |8.80 | ||
| | |||
|-- | |-- | ||
|Red | |Red | ||
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|1.53 | |1.53 | ||
|1.61 | |1.61 | ||
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|-- | |-- | ||
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Revision as of 18:39, 5 April 2009
T/R Luciferase Reactions
Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (Renilla/firefly).
Note: feel free to put ratios from either background-subtracted or raw values; they are likely to be quite similar. Hoewver, if you see this note, please mark which kind of data your ratios come from.
| Team Colour | none | pLuc only | validated | scrambled | experimental | subtracted or raw? | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | ||
| Pink | 4.5 | 4.0 | 20.7 | 19.1 | 2.73 | 1.92 | 18.9 | 22.9 | 14.7 | 17.0 | |
| Green | .94 | .97 | 36.4 | 26.3 | 2.7 | 2.4 | 27.6 | 29.1 | 2.8 | 2.6 | |
| Orange | .076 | 22.3 | 21.2 | 29.0 | 2.25 | 1.74 | 22.0 | 22.8 | 4.8 | 4.1 | |
| Yellow | 1.33 | .17 | 20.8 | 29.4 | 1.42 | 2.24 | 25.74 | 26.08 | 1.17 | 1.53 | |
| Grey | 1.74 | 1.10 | 38.64 | 34.59 | 2.55 | 2.90 | 37.75 | 35.83 | 9.04 | 8.80 | |
| Red | 2.02 | 3.00 | 21.36 | 16.40 | 1.31 | 1.51 | 20.78 | 23.90 | 1.53 | 1.61 | |
W/F Luciferase Reactions
Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (Renilla/firefly).
| Team Colour | none | pLuc only | validated | scrambled | experimental | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | |
| Pink | 1.83 | 1.39 | 22.59 | 20.86 | 2.87 | 2.91 | 21.06 | 21.61 | 18.10 | 18.08 |
| Orange | 1.11 | 0.89 | 20.56 | 11.30 | 1.70 | 2.30 | 21.71 | 16.40 | 6.42 | 5.13 |
| Red | 2.133 | 2.6 | 5.924 | 14.4 | 0.385 | 1.636 | 20.96 | 19.89 | 1.96 | 1.72 |
| Blue | 1.019 | 0.761658 | 16.17009 | 19.39064 | 2.758433 | 8.679711 | 15.43884 | 10.86473 | 14.8144 | 12.87329 |
