Talk:20.109(S09): Luciferase assays and RNA prep (Day6): Difference between revisions
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| Line 145: | Line 145: | ||
| Rep 2 ratio | | Rep 2 ratio | ||
|-- | |-- | ||
| | |Pink | ||
| | |1.83 | ||
| | |1.39 | ||
| | |22.59 | ||
| | |20.86 | ||
| | |2.87 | ||
| | |2.91 | ||
| | |21.06 | ||
| | |21.61 | ||
| | |18.10 | ||
| | |18.08 | ||
|-- | |-- | ||
| | | | ||
Revision as of 12:20, 3 April 2009
T/R Luciferase Reactions
Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (Renilla/firefly).
| Team Colour | none | pLuc only | validated | scrambled | experimental | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | |
| Pink | 4.5 | 4.0 | 20.7 | 19.1 | 2.73 | 1.92 | 18.9 | 22.9 | 14.7 | 17.0 |
W/F Luciferase Reactions
Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (Renilla/firefly).
| Team Colour | none | pLuc only | validated | scrambled | experimental | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | |
| Pink | 1.83 | 1.39 | 22.59 | 20.86 | 2.87 | 2.91 | 21.06 | 21.61 | 18.10 | 18.08 |
