Talk:20.109(S09):Data analysis (Day8): Difference between revisions
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| Line 191: | Line 191: | ||
|E31R | |E31R | ||
|Kd~0.49 μM, n~0.96 | |Kd~0.49 μM, n~0.96 | ||
|Used Teaching Sample: | |Used Teaching Sample: Kd~0.5 μM, n~1.1 | ||
|Kd~0.45 μM, n~7.4 | |Kd~0.45 μM, n~7.4 | ||
|E31R decreased calcium binding and cooperativity | |E31R decreased calcium binding and cooperativity | ||
Revision as of 20:07, 7 March 2009
T/R data
Below is your calcium titration data, as well as data from pilot experiments done by the teaching faculty for comparison.
Please fill in the second table by Sunday, if not today, so your classmates can compare their data to yours.
| Day 7 data file | Data taken after 24 hr settling period |
| Media:S09_M1D7_TeachingExample.xls | Mutations: WT, S101L, Y64D |
| Media:S09_M1D7_TR_Blue.txt | |
| Media:S09_M1D7_TR_Green.txt | Media:S09_M1D7_TR_Green-redo.txt |
| Media:S09_M1D7_TR_Grey.txt | Media:S09_M1D7_TR_Grey-redo.txt |
| Media:S09_M1D7_TR_Orange.txt | |
| Media:S09_M1D7_TR_Pink.txt | Media:S09_M1D7_TR_Pink-redo.txt |
| Media:S09_M1D7_TR_Purple.txt | Media:S09_M1D7_TR_Purple-redo.txt |
| Media:S09_M1D7_TR_Red.txt | |
| Media:S09_M1D7_TR_Yellow.txt |
| Group Colour | X#Z Mutant | Most believable KD; n for X#Z mutant | Most believable KD; n for S101L | Most believable KD; n for WT | Comments |
| Teaching Faculty | Y64D | N/A | ~ 0.5 μM ; ~ 8 | ~ 0.3 μM; ~ 1(.2) | Y64D killed calcium response |
| Yellow | Y64D | N/A | ~0.4 μM; ~7 | ~0.5 μM; ~3 | No Calcium binding in Y64D |
| Grey | D22W | N/A | 0.46 μM ; ~ 8 | 0.3 μM; ~ 2 | killed calcium binding |
W/F data
Below is your calcium titration data, as well as data from pilot experiments done by the teaching faculty for comparison.
Please fill in the second table by Sunday, if not today, so your classmates can compare their data to yours.
| Group Colour | X#Z Mutant | Most believable KD; n for X#Z mutant | Most believable KD; n for S101L | Most believable KD; n for WT | Comments |
| Teaching Faculty | Y64D | N/A | ~ 0.5 μM ; ~ 8 | ~ 0.3 μM ; ~ 1(.2) | Y64D killed calcium response |
| Orange | E127K | Kd-.4558, n-1.2418 | N/A (using teaching data) | Kd=.6285, n=.1490 | S101L and E127K data was noisy |
| Green | T79P | Kd~0.84 μM, n~2.5 | Kd~0.52 μM, n~7.5 | Kd~0.50 μM, n~7.6 | T79P seems to have decreased cooperativity |
| Blue | E31R | Kd~0.49 μM, n~0.96 | Used Teaching Sample: Kd~0.5 μM, n~1.1 | Kd~0.45 μM, n~7.4 | E31R decreased calcium binding and cooperativity |
