Talk:20.109(S08):Testing cell viability (Day3): Difference between revisions
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|4:35-5:00 | |4:35-5:00 | ||
|3:05 | |3:05 | ||
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|3:45-4:10 | |3:45-4:10 | ||
|2:15 | |2:15 | ||
| | |Green | ||
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|4:10-4:35 | |4:10-4:35 | ||
|2:40 | |2:40 | ||
| | |Pink | ||
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|4:35-5:00 | |4:35-5:00 | ||
|3:05 | |3:05 | ||
| | |Yellow | ||
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Please post one picture for each of your two alginate samples (you can post just the green channel). | Please post one picture for each of your two alginate samples (you can post just the green channel). | ||
[[Image:20.109_live-dead-example.png|thumb|Agi's example pictures, 40X]] | [[Image:20.109_live-dead-example.png|thumb|Caption for Agi's example pictures, 40X, used 1% alginate]] | ||
[[Image:Yellow1.jpg|thumb|Figure: Yellow Group. 10X FITC filter. 3D 1% alginate chondrocyte cells with no collagen II.]] | |||
<br style="clear:both;"/> | |||
[[Image:DSCF1329.jpg|thumb|right|Figure: Pink Group. 10X FITC filter. 3D 1% alginate chondrocyte cells with no toxin added.]] | |||
[[Image:DSCF1332.jpg|thumb|left|Figure: Pink Group. 10X FITC filter. 3D 1% alginate chondrocyte cells with toxin added.]] | |||
[[Image:DSCF1334.jpg|thumb|center|Figure: Pink Group. 10X FITC filter. 2D chondrocyte cells with rich media.]] | |||
[[Image:2D-3.jpg|thumb|right|Figure: Red Group. 10X FITC filter. 2D chondrocyte cells with media w/o ascorbate.]] | |||
[[Image:3D-Lo3.jpg|thumb|left|Figure: Red Group. 10X FITC filter. 3D 2% Sigma Aldrich alginate in rich media.]] | |||
[[Image:20.109S08_TRPurple_2D_Samp.jpg|thumb|center|Figure: Purple Group. 10X FITC filter. 2D sample.]] | |||
[[Image:GreenTR2D.jpg|thumb|right|Figure: Green Group. 10X FITC filter. 2D sample.]] | |||
[[Image:GreenTR3D5million.jpg|thumb|left|Figure: Green Group. 10X FITC filter. 3D sample. 5million in 1%alginate]] | |||
[[Image:GreenTR3D20million.jpg|thumb|center|Figure: Green Group. 10X FITC filter. 2D sample. 20million in 1%alginate]] | |||
[[Image:Figure-_Blue_Group.|thumb|Figure: Blue Group. 10X FITC filter. 2D sample. 1million cells]] | |||
<br style="clear:both;"/> | |||
==W/F pictures== | ==W/F pictures== | ||
Please post one picture for each of your two alginate samples (you can post just the green channel). | |||
[[Image:20.109_live-dead-example.png|thumb|Caption for Agi's example pictures, 40X, used 1% alginate]] | |||
[[Image:mod3day4 figs.png|thumb|right|Purple Group. Left Figure: 10X FITC Filter, 2D Sample. Right Figure: 10X EthD-1 Filter, 3D Sample in 1% alginate]] | |||
[[Image:DSCF1378.JPG|thumb|left|Red Group. Left figure: 10X FITC filter, 3D with mechanical stress, 1% alginate. Right figure: 10X FITC Filter, 2D 1% alginate]] | |||
[[Image:3d control live.jpg|thumb|center|Blue Group. 10X FITC filter, 3D normal calcium concentration, 2% alginate]] | |||
[[Image:3d control dead.jpg|thumb|right|Blue Group. 10X EthD-1 Filter, 3D normal calcium concentration, 2% alginate]] | |||
[[Image:2D good.jpg|thumb|left|Green Group. 10X FITC filter, 2D normal culture.]][[Image:3D good.jpg|thumb|Green Group. 10X FITC filter, 3D 2% Sigma brand low viscosity alginate culture.]] | |||
[[Image:3D CO -1.JPG|thumb|center| Pink Group. 10X FITC filter, control culture in 1% alginate. ]] | |||
[[Image:3D Weight -1.JPG|thumb| Pink Group. 10X FITC filter, weighted culture in 1% alginate. Weights were used to test response of chondrocyes to mechanical stress.]] | |||
[[Image:2D_fluorescent_(red_green_10X).jpg|thumb|right| Yellow Group. 2D sample 10X ]] | |||
[[Image:2D_fluorescent_(red_green_40X).jpg|thumb|left| Yellow Group. 2D sample 40X ]] | |||
[[Image:3D_fluorescent_(red_green_10X).jpg|thumb|center| Yellow Group. 3D sample 10X ]] | |||
<br style="clear:both;"/> | |||
Latest revision as of 07:08, 26 April 2008
Please write your team colour next to the spot that you would like.
Feel free to arrive earlier than your scheduled time!
T/R schedule
| Microscope time | Arrival time (at latest!) | Team colour |
| 2:30-2:55 | 1:05 | Yellow |
| 2:55-3:20 | 1:25 | Pink |
| 3:20-3:45 | 1:50 | Red |
| 3:45-4:10 | 2:15 | Purple |
| 4:10-4:35 | 2:40 | Green |
| 4:35-5:00 | 3:05 | Blue |
W/F schedule
| Microscope time | Arrival time (at latest!) | Team colour |
| 2:30-2:55 | 1:05 | Purple |
| 2:55-3:20 | 1:25 | Red |
| 3:20-3:45 | 1:50 | Blue |
| 3:45-4:10 | 2:15 | Green |
| 4:10-4:35 | 2:40 | Pink |
| 4:35-5:00 | 3:05 | Yellow |
T/R pictures
Please post one picture for each of your two alginate samples (you can post just the green channel).
W/F pictures
Please post one picture for each of your two alginate samples (you can post just the green channel).
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