Talk:20.109(S08):Testing cell viability (Day3)

Please write your team colour next to the spot that you would like.

Feel free to arrive earlier than your scheduled time!

T/R schedule

Microscope time	Arrival time (at latest!)	Team colour
2:30-2:55	1:05	Yellow
2:55-3:20	1:25	Pink
3:20-3:45	1:50	Red
3:45-4:10	2:15	Purple
4:10-4:35	2:40	Green
4:35-5:00	3:05	Blue

W/F schedule

Microscope time	Arrival time (at latest!)	Team colour
2:30-2:55	1:05	Purple
2:55-3:20	1:25	Red
3:20-3:45	1:50	Blue
3:45-4:10	2:15	Green
4:10-4:35	2:40	Pink
4:35-5:00	3:05	Yellow

T/R pictures

Please post one picture for each of your two alginate samples (you can post just the green channel).

Caption for Agi's example pictures, 40X, used 1% alginate

Figure: Yellow Group. 10X FITC filter. 3D 1% alginate chondrocyte cells with no collagen II.

Figure: Pink Group. 10X FITC filter. 3D 1% alginate chondrocyte cells with no toxin added.

Figure: Pink Group. 10X FITC filter. 3D 1% alginate chondrocyte cells with toxin added.

Figure: Pink Group. 10X FITC filter. 2D chondrocyte cells with rich media.

Figure: Red Group. 10X FITC filter. 2D chondrocyte cells with media w/o ascorbate.

Figure: Red Group. 10X FITC filter. 3D 2% Sigma Aldrich alginate in rich media.

Figure: Purple Group. 10X FITC filter. 2D sample.

Figure: Green Group. 10X FITC filter. 2D sample.

Figure: Green Group. 10X FITC filter. 3D sample. 5million in 1%alginate

Figure: Green Group. 10X FITC filter. 2D sample. 20million in 1%alginate

Figure: Blue Group. 10X FITC filter. 2D sample. 1million cells

W/F pictures

Please post one picture for each of your two alginate samples (you can post just the green channel).

Caption for Agi's example pictures, 40X, used 1% alginate

Purple Group. Left Figure: 10X FITC Filter, 2D Sample. Right Figure: 10X EthD-1 Filter, 3D Sample in 1% alginate

Red Group. Left figure: 10X FITC filter, 3D with mechanical stress, 1% alginate. Right figure: 10X FITC Filter, 2D 1% alginate

Blue Group. 10X FITC filter, 3D normal calcium concentration, 2% alginate

Blue Group. 10X EthD-1 Filter, 3D normal calcium concentration, 2% alginate

Green Group. 10X FITC filter, 2D normal culture.

Green Group. 10X FITC filter, 3D 2% Sigma brand low viscosity alginate culture.

Pink Group. 10X FITC filter, control culture in 1% alginate.

Pink Group. 10X FITC filter, weighted culture in 1% alginate. Weights were used to test response of chondrocyes to mechanical stress.

Yellow Group. 2D sample 10X

Yellow Group. 2D sample 40X

Yellow Group. 3D sample 10X