Talk:20.109(S08):DNA engineering/Restriction map and tissue culture (Day 6)
From OpenWetWare
Diagnostic Digests
TR lab
Many of the gels today did not have a visible ladder (the protocol was an old one that said to use only 5 μL - sorry about that). A few gels - for blue, and to a large extent purple and red - also seemed to indicate little DNA recovery from the minipreps.
Team colour | Digest 1 | Digest 2 |
Green | XhoI+EcoRV | BamHI+SalI |
Purple | ||
Red | EcoRV+NotI | BamHI+XhoI |
Pink | SalI+EcoRV | NotI+EcoRV |
Blue | NotI+EcoRV | XhoI+BamHI |
Yellow | BamH1+Sal1 | EcoRV+Nsi1 |
WF lab
The gels were pretty bright today, including the ladder. For the blue group, the MW ladder is only faintly/partly visible on the original polaroid. The bands seem to fall b/w 4-5 Kbp for the most part, but it's hard to say for sure.
Team colour | Digest 1 | Digest 2 |
Green | write here | etc. |
Purple | ||
Red | ||
Pink | BamHI/SalI | BamHI/NsiI |
Blue | BamHI/XhoI | Nsi1/EcoRV |
Yellow |