Talk:20.109(S08):DNA engineering/Restriction map and tissue culture (Day 6)

Diagnostic Digests

TR lab

Many of the gels today did not have a visible ladder (the protocol was an old one that said to use only 5 μL - sorry about that). A few gels - for blue, and to a large extent purple and red - also seemed to indicate little DNA recovery from the minipreps.

Tue/Thu gels, 3 of 3

Tue/Thu gels, 1 of 3

Tue/Thu gels, 2 of 3

Team colour	Digest 1	Digest 2
Green	XhoI+EcoRV	BamHI+SalI
Purple
Red	EcoRV+NotI	BamHI+XhoI
Pink	SalI+EcoRV	NotI+EcoRV
Blue	NotI+EcoRV	XhoI+BamHI
Yellow	BamH1+Sal1	EcoRV+Nsi1

WF lab

The gels were pretty bright today, including the ladder. For the blue group, the MW ladder is only faintly/partly visible on the original polaroid. The bands seem to fall b/w 4-5 Kbp for the most part, but it's hard to say for sure.

Team colour	Digest 1	Digest 2
Green	write here	etc.
Purple
Red
Pink	BamHI/SalI	BamHI/NsiI
Blue	BamHI/XhoI	Nsi1/EcoRV
Yellow

Wed/Fri gels, 1 of 2

Wed/Fri gels, 2 of 2