Talk:20.109(S07): Measuring calcium in vitro: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
Line 111: | Line 111: | ||
[[Image: RFU,Ca2+_WFblue.jpeg|thumb|left| W\F Blue Team]] | [[Image: RFU,Ca2+_WFblue.jpeg|thumb|left| W\F Blue Team]] | ||
[[Image: RFU_peak_WF_green.jpg|thumb|left| W\F Green Team]] | [[Image: RFU_peak_WF_green.jpg|thumb|left| W\F Green Team]] | ||
[[Image: rfu vs ca2+.jpeg|thub|left| T/R Blue Team]] |
Revision as of 21:31, 11 March 2007
Data summary for T/R lab section
Team Color | equation for standard's best-fit line | R-squared | unknown solution tested | concentration (triplicate), units | average concentration |
---|---|---|---|---|---|
Red | |||||
Blue | |||||
Purple | |||||
Yellow | |||||
Green | Absorbance = 0.1002([CaCl2]) | R^2 = 0.9644 | Embryonic Stem Cell Medium | [Ca2+] = 3.045, 1.906, and 2.076 (mM) | Average [Ca2+] = 2.342 mM |
Pink |
Data summary for W/F lab section
Team Color | equation for standard's best-fit line | R-squared | unknown solution tested | concentration (triplicate), units | average concentration |
---|---|---|---|---|---|
Red | y = 0.1419x - 0.0076 | R2 = 0.9972 | ESM | 1.9098, 2.4489, 1.7900, mM | 2.0496 mM |
Blue | ABS = (8*10^-5)[Ca2+] + 0.1633, with [Ca2+] in microM | R^2 = .9665 | trypsin | [negative/0] | [negative/0] |
Purple | |||||
Yellow | |||||
Green | ABS=0.1037*(Ca conc. in mM) | R^2=0.9891 | media | 2.266, 2.011, 2.170 mM | 2.149 mM |
Pink |