Talk:20.109(F14): Mod 2 Day 7 Readout of Protein: Difference between revisions
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==Strains #s and Mutations== | |||
*466 = wt | |||
*468 = Kinase dead | |||
*486 = T541R | |||
*487 = T541Y | |||
*488 = T541P | |||
*489 = T541R | |||
*491 = T541L | |||
*492 = T541silent change | |||
==T/R b-gal data== | ==T/R b-gal data== | ||
Be sure to add the mutant strain # when you fill in this chart! | Be sure to add the mutant strain # when you fill in this chart! | ||
Revision as of 13:56, 4 November 2014
Strains #s and Mutations
- 466 = wt
- 468 = Kinase dead
- 486 = T541R
- 487 = T541Y
- 488 = T541P
- 489 = T541R
- 491 = T541L
- 492 = T541silent change
T/R b-gal data
Be sure to add the mutant strain # when you fill in this chart!
| Team | Strain | OD600 (1:10) | OD600 | Elapsed time (minutes) | Abs420 (list both values) | OD550 (list both values) | Units |
| Cherries | WT | ||||||
| KD | |||||||
| mut1: 487 | .27 | 2.7 | 18.83 light, 19 dark | .552 light, .587 dark | .0045 light, .0045 dark | 1070 light, 1218 dark | |
| mut2: 491 | .25 | 2.5 | 19.83 light, 19.83 dark | .670 light, .608 dark | .0075 light, .006 dark | 1273 light, 1255 dark |
| Team | Strain | OD600 (1:10) | OD600 | Elapsed time (minutes) | Abs420 (list both values) | OD550 (list both values) | Units |
| Orange | WT | ||||||
| KD | |||||||
| mut1 | |||||||
| mut2 |
| Team | Strain | OD600 (1:10) | OD600 | Elapsed time (minutes) | Abs420 (list both values) | OD550 (list both values) | Units |
| Yellow | WT | ||||||
| KD | |||||||
| mut1 | |||||||
| mut2 |
| Team | Strain | OD600 (1:10) | OD600 | Elapsed time (minutes) | Abs420 (list both values) | OD550 (list both values) | Units |
| Green | WT | ||||||
| KD | |||||||
| mut1 | |||||||
| mut2 |
| Team | Strain | OD600 (1:10) | OD600 | Elapsed time (minutes) | Abs420 (list both values) | OD550 (list both values) | Units |
| Blue | WT | ||||||
| KD | |||||||
| mut1 | |||||||
| mut2 |
| Team | Strain | OD600 (1:10) | OD600 | Elapsed time (minutes) | Abs420 (list both values) | OD550 (list both values) | Units |
| Pink | WT | ||||||
| KD | |||||||
| mut1 | |||||||
| mut2 |
| Team | Strain | OD600 (1:10) | OD600 | Elapsed time (minutes) | Abs420 (list both values) | OD550 (list both values) | Units |
| Purple | WT | ||||||
| KD | |||||||
| mut1 | |||||||
| mut2 |
W/F b-gal data
Be sure to add the mutant strain # when you fill in this chart!
| Team | Strain | OD600 (1:10) | OD600 | Elapsed time (minutes) | Abs420 (list both values) | OD550 (list both values) | Units |
| Green | WT | ||||||
| KD | |||||||
| mut1 | |||||||
| mut2 |
| Team | Strain | OD600 (1:10) | OD600 | Elapsed time (minutes) | Abs420 (list both values) | OD550 (list both values) | Units |
| Blue | WT | ||||||
| KD | |||||||
| mut1 | |||||||
| mut2 |
| Team | Strain | OD600 (1:10) | OD600 | Elapsed time (minutes) | Abs420 (list both values) | OD550 (list both values) | Units |
| Pink | WT | ||||||
| KD | |||||||
| mut1 | |||||||
| mut2 |
